Log In Sign up
The largest database of trusted experimental protocols

Mouse anti irf4 ab

Manufactured by Thermo Fisher Scientific
Visit Supplier

Mouse anti-IRF4 Ab is an antibody that binds to the Interferon Regulatory Factor 4 (IRF4) protein. IRF4 is a transcription factor involved in regulating immune cell development and function. This antibody can be used for the detection and study of IRF4 in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Can get signal immunoreaction enhancer solution, by Toyobo (2 mentions) Alexa546 conjugated anti mouse igg ab, by Thermo Fisher Scientific (2 mentions) Goat anti irf4 ab, by Santa Cruz Biotechnology (2 mentions) Rabbit anti rick ab, by Abcam (2 mentions) Rabbit anti myd88 ab, by Abcam (2 mentions) Rabbit anti traf6 ab, by Abcam (2 mentions) Duolink in situ kit, by Olink (2 mentions)

2 protocols using mouse anti irf4 ab

1

Visualizing IRF4 Interactions in Colon Samples

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Colon tissues were harvested and fixed in 10% formalin. Deparaffinized colon sections were then obtained and incubated with mouse anti-IRF4 Ab (Invitrogen) followed by Alexa546-conjugated anti-mouse IgG Ab (Invitrogen) by using Can Get Signal immunoreaction enhancer solution (Toyobo) as described previously 43 (link). For visualization of interaction between IRF4 and RICK, MyD88, TRAF6 in colon samples, a Duolink In Situ kit was used (Olink Bioscience). Goat anti-IRF4 Ab (Santa Cruz), rabbit anti-RICK Ab, rabbit anti-MyD88 Ab, and rabbit anti-TRAF6 Ab (Abcam) were used to visualize target protein interactions. For visualization of interaction between IRF4 and RICK, MyD88, TRAF6 in human DCs, cells were fixed in 4% paraformaldehyde and subjected to the protocols suggested by the Duolink In Situ kit.
Watanabe T., Asano N., Meng G., Yamashita K., Arai Y., Sakurai T., Kudo M., Fuss I.J., Kitani A., Shimosegawa T., Chiba T, & Strober W. (2014). NOD2 Down-Regulates Colonic Inflammation by IRF4-Mediated Inhibition of K63-Linked Polyubiquitination of RICK and TRAF6. Mucosal immunology, 7(6), 1312-1325.
+ Open protocol
+ Expand
2

Visualizing IRF4 Interactions in Colon Samples

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Colon tissues were harvested and fixed in 10% formalin. Deparaffinized colon sections were then obtained and incubated with mouse anti-IRF4 Ab (Invitrogen) followed by Alexa546-conjugated anti-mouse IgG Ab (Invitrogen) by using Can Get Signal immunoreaction enhancer solution (Toyobo) as described previously 43 (link). For visualization of interaction between IRF4 and RICK, MyD88, TRAF6 in colon samples, a Duolink In Situ kit was used (Olink Bioscience). Goat anti-IRF4 Ab (Santa Cruz), rabbit anti-RICK Ab, rabbit anti-MyD88 Ab, and rabbit anti-TRAF6 Ab (Abcam) were used to visualize target protein interactions. For visualization of interaction between IRF4 and RICK, MyD88, TRAF6 in human DCs, cells were fixed in 4% paraformaldehyde and subjected to the protocols suggested by the Duolink In Situ kit.
Watanabe T., Asano N., Meng G., Yamashita K., Arai Y., Sakurai T., Kudo M., Fuss I.J., Kitani A., Shimosegawa T., Chiba T, & Strober W. (2014). NOD2 Down-Regulates Colonic Inflammation by IRF4-Mediated Inhibition of K63-Linked Polyubiquitination of RICK and TRAF6. Mucosal immunology, 7(6), 1312-1325.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!