Panc 1 pancreatic ductal adenocarcinoma cells

Dithiothreitol (DTT), PNGase F and sequencing grade trypsin were from Promega (Madison, WI). Optimal LC/MS grade acetonitrile (ACN), methanol (MeOH) and water were from Fisher Scientific (Pittsburgh, PA). Concanavalin A (ConA), wheat germ agglutinin (WGA), Ricinuscommunis agglutinin (RCA120), iodoacetamide (IAA), acetyl-D18 glucosamine, D-lactose, methyl α-D-mannopyranoside and manganese dichloride were obtained from Sigma-Aldrich (St. Louis, MO). Tris base, urea (UA), sodium chloride, ammonium bicarbonate (ABC) and calcium chloride (CaCl₂) were obtained from Fisher Scientific (Pittsburgh, PA). Trifluoroacetic acid (TFA), triethylammonium bicarbonate (TEAB), N, N-dimethylformamide (DMF), 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium tetrafluoroborate (DMTMM), N-methylmorpholine (NMM) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO). Hydroxylamine solution was purchased from Alfa Aesar (Ward Hill, MA). C18 OMIX tips were obtained from Agilent (Santa Clara, CA). Hydrophilic interaction chromatography material (PolyHYDROXYETHYL A) was obtained from PolyLC (Columbia, MD). Microcon filters YM-30 (30 kDa) was purchased from Merck Millipore (Billerica, MA). PANC-1 pancreatic ductal adenocarcinoma cells were from ATCC (Manassas, VA). Duplex DiLeu tags were custom synthesized in our own lab.^{45 (link)}