For immunofluorescent stains, we rinsed the cells and 3D cultures twice with PBS (phosphate buffered saline). Cells were then fixed through a RT 15–30 min incubation in fresh 4% paraformaldehyde aqueous solution (157–4, ElectronMicroscopy Sciences) followed by rinsing twice with PBS. Cells were permeabilized through incubation in 0.1% Triton X-100 in PBST (phosphate buffered saline with 0.1% tween®20) for 15 minutes at RT. Cell on-specific binding was blocked through overnight incubation in 3% human serum albumin in PBST at 4 °C. After a 24-hours incubation with the primary antibody solutions at 4°C, the cells were washed five times. The following antibodies (and dilutions) were used: anti-p-tau antibody (1:40, AT8, Thermo Scientific, MN1020); anti-PHF (1:1000, A gift from P. Davies, Albert Einstein College of Medicine); anti-GFAP antibody (1:500, Neuromap, N206A/8), anti-MAP2 antibody (1:200, Cell Signaling Technology, 4542); anti-CD68 (1:100, Cell Signaling, 76437); anti-cd11b (1:100, Life Technologies, NB110–89474); anti-S100 (1:400, Abcam, ab868); anti-S100A6 (1:200, Cell Signaling, D3H3W); anti-S100B (1:500, Abcam, Ab218515); anti-ALDH1L1 (1:200, EMD Millipore, MABN495).
Anti p tau antibody
Manufactured by Thermo Fisher Scientific
The Anti-p-tau antibody is a laboratory tool used to detect and measure the presence of phosphorylated tau protein in samples. Phosphorylated tau is a biomarker associated with certain neurodegenerative diseases. The antibody can be used in various analytical techniques, such as immunoassays, to quantify the levels of this specific protein in research samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti s100, by Abcam (2 mentions)
Anti map2 antibody, by Cell Signaling Technology (2 mentions)
Anti aldh1l1, by Merck Group (2 mentions)
Anti s100b, by Abcam (2 mentions)
Anti cd11b, by Thermo Fisher Scientific (2 mentions)
Anti cd68, by Cell Signaling Technology (2 mentions)
Paraformaldehyde aqueous solution, by Electron Microscopy Sciences (2 mentions)
Anti s100a6, by Cell Signaling Technology (2 mentions)
2 protocols using anti p tau antibody
1
Immunofluorescent Staining of Cells and 3D Cultures
2
Immunofluorescent Staining of Cells and 3D Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For immunofluorescent stains, we rinsed the cells and 3D cultures twice with PBS (phosphate buffered saline). Cells were then fixed through a RT 15–30 min incubation in fresh 4% paraformaldehyde aqueous solution (157–4, ElectronMicroscopy Sciences) followed by rinsing twice with PBS. Cells were permeabilized through incubation in 0.1% Triton X-100 in PBST (phosphate buffered saline with 0.1% tween®20) for 15 minutes at RT. Cell on-specific binding was blocked through overnight incubation in 3% human serum albumin in PBST at 4 °C. After a 24-hours incubation with the primary antibody solutions at 4°C, the cells were washed five times. The following antibodies (and dilutions) were used: anti-p-tau antibody (1:40, AT8, Thermo Scientific, MN1020); anti-PHF (1:1000, A gift from P. Davies, Albert Einstein College of Medicine); anti-GFAP antibody (1:500, Neuromap, N206A/8), anti-MAP2 antibody (1:200, Cell Signaling Technology, 4542); anti-CD68 (1:100, Cell Signaling, 76437); anti-cd11b (1:100, Life Technologies, NB110–89474); anti-S100 (1:400, Abcam, ab868); anti-S100A6 (1:200, Cell Signaling, D3H3W); anti-S100B (1:500, Abcam, Ab218515); anti-ALDH1L1 (1:200, EMD Millipore, MABN495).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!