To allow time for optimal expression of ArchT, tetrodes were advanced to the dorsal CA1 region over a minimum period of four weeks. The positions of tetrodes were estimated using turn counts (282 µm per turn) and electrophysiological features characteristic of CA1 including the presence of theta oscillations, sharp-wave ripple events and theta-modulated complex spiking activity. Signals were amplified 4000–8000 times, bandpass filtered between 400 Hz and 8000 Hz to capture spike activity and digitized at 40 kHz by an Omniplex Neural Acquisition system (Plexon, Inc.). Position data was captured using LEDs situated on the rat’s head stage that were monitored at 30 Hz by a Cineplex Digital Capture System (Plexon, Inc.) and synchronized to neural data.
Omniplex neural acquisition system
Manufactured by Plexon
Sourced in United States
The Omniplex Neural Acquisition System is a data acquisition device designed for recording and analyzing neural signals. It is capable of simultaneously recording from multiple electrodes and provides a flexible platform for neural data collection and processing.
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Lab products found in correlation
2 protocols using omniplex neural acquisition system
1
Tetrode Positioning and Recording in Rat CA1
2
Neural Signal Acquisition and Analysis
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Following construction and curing of the surgical head cap, recordings for a period of 10 min were collected using an OmniPlex Neural Acquisition System (Plexon Inc., Dallas, TX, USA) connected to the a-SiC array via Omnetics connector and a 16-channel digital headstage. Wideband signals (0.1–7000 Hz) were recorded simultaneously from all 16 electrodes at 40 kHz sampling frequency and later filtered offline using a 4-pole Butterworth high pass filter (250 Hz). A −4σ threshold based on RMS noise calculations was applied to filtered continuous data to identify potential waveforms (or spikes). Single units were identified manually based on 2D principal component clustering using Plexon’s Offline Sorter software (Plexon, Dallas, TX, USA). Sorted units which were not comprised of at least 100 individual spikes or which exhibited greater than 0.5% spike refractory period violations were excluded from analysis. Signal-to-noise ratios (SNR) were calculated by dividing the mean peak-to-peak amplitude of each unit by the adjusted RMS noise of the associated channel, which excluded values greater or less than ±4σ of the filtered continuous signal.
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