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Nck1/2 is a family of adapter proteins that facilitate signal transduction by binding to activated receptor tyrosine kinases and other signaling molecules. They play a role in the regulation of cytoskeletal organization, cell migration, and other cellular processes.

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Lab products found in correlation

Anti mnanog, by R&D Systems (1 mentions) Peif4eser209, by Cell Signaling Technology (1 mentions) Anti pkr, by Santa Cruz Biotechnology (1 mentions) Anti eif2α, by Cell Signaling Technology (1 mentions) Icam 1, by Cell Signaling Technology (1 mentions) Integrin α5, by Santa Cruz Biotechnology (1 mentions) Erk1 2, by Santa Cruz Biotechnology (1 mentions) Vcam 1, by Santa Cruz Biotechnology (1 mentions)

2 protocols using nck1 2

1

Molecular Markers of Stem Cell Identity

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Anti-eIF2α, P-eIF2α (Ser51), eIF4G, P-eIF4G (Ser1108), eIF4E, P-eIF4E (Ser209) and Oct4 antibodies were obtained from Cell Signaling Technology (Danvers, MA). Anti-mNanog was obtained from R&D Systems (Minneapolis, MN). Anti-hNANOG and pPKR (Thr446) were obtained from Millipore (Billerica, MA), and anti-Actin (mouse C4) and c-Myc (polyclonal) were obtained from Sigma (St. Louis, MO). Anti-PKR and Nck1/2 were obtained from Santa Cruz Biotechnology (Dallas, TX). Anti-CReP was obtained from ProteinTech (Chicago, IL).
Friend K., Brooks H.A., Propson N.E., Thomson J.A, & Kimble J. (2015). Embryonic Stem Cell Growth Factors Regulate eIF2α Phosphorylation. PLoS ONE, 10(9), e0139076.
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2

Quantitative Western Blot Analysis

Cited 2 times
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Western blotting was performed as previously described (20 (link), 28 (link)). Primary antibodies included anti-PAK2, phospho-NF-κB (p65 subunit, Ser536), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ICAM-1 (Cell Signaling Technology), phosphotyrosine (4G10), phospho-PAK1/2/3 (Ser141; Invitrogen), Nck1/2, p65, VCAM-1, α5 integrin, and ERK1/2 (Santa Cruz Biotechnology). Nck1 and Nck2 specific antibodies were kindly provided by Dr. Louise Larose (McGill University) (51 (link)). Densitometry was performed using ImageJ software.
Chen J., Leskov I.L., Yurdagul A J.r., Thiel B., Kevil C.G., Stokes K.Y, & Orr A.W. (2015). Recruitment of the adaptor protein Nck to PECAM-1 couples oxidant stress to canonical NF-κB signaling and inflammation. Science signaling, 8(365), ra20.
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