GlcNAc-PEI was synthesized according to a previous report with slight modifications30 (link). Briefly, chitobiose (0.1 g) was dissolved in 20% aqueous methanol and was mixed with 0. 285 g of iodine. Next, 1 mol/L of potassium hydroxide solution was slowly dropped into the mixture until the iodine color completely disappeared. Thereafter, the solution was concentrated in vacuo to produce crude chitobionic acid. The 25 mol% chitobionic acid solution was then reacted with PEI-1800 (Sigma-Aldrich, St Louis, MO, USA) using water-soluble carbodiimide [1-ethyl-3-3(-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Sigma-Aldrich, St Louis, MO, USA) as a coupling agent, under stirring at room temperature. After stirring at room temperature for 24 h, the solution was dialyzed for 48 h using a dialysis membrane with a cutoff of 2500 Da.
Pei 1800
Manufactured by Merck Group
Sourced in United States
PEI-1800 is a high-molecular-weight polyethylenimine (PEI) product developed by Merck Group for use in various laboratory applications. It is a cationic polymer with a molecular weight of approximately 1,800 kDa. PEI-1800 can be used as a transfection reagent for the delivery of nucleic acids into cells.
Automatically generated - may contain errors
Lab products found in correlation
Anhydrous ethylene dichloride, by Merck Group (1 mentions)
Milli q instrument, by Merck Group (1 mentions)
Endofree plasmid maxi, by Qiagen (1 mentions)
Cellulose membrane, by Thermo Fisher Scientific (1 mentions)
High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
2 protocols using pei 1800
1
Synthesis of GlcNAc-PEI Conjugate
2
Synthesis and Characterization of PEI-PDA Nanoparticles
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PEI 1800 and 25 kDa were purchased from Sigma-Aldrich. Anhydrous ethylene dichloride was obtained from Sigma-Aldrich. 2,6-pyridinedicarboxaldehyde (PDA) was purchased from TCI (Shanghai) Development Co., Ltd. Cellulose membranes (MWCO = 10,000 Da) were purchased from Thermo Scientific. Water was purified using a milli-Q instrument (Millipore). Poly (ethylene glycol) (PEG) standards kit (ranging from 106 to 20100 Da in molecular weight) was purchased from Polymer Standards Service GmbH. All the reagents were used without further purification.
Plasmid DNA encoding GFP and mouse-VEGF-A siRNA expression vector was constructed from pGPU6/GFP/Neo vector (Bioroot Biology, Shanghai, China) according to a previous report. The targeted VEGF-A sequences were 5′- CGATGAAGCCCTGGAGTGC -3′. And the plasmid was amplified using EndoFreeTM Plasmid Maxi (Qiagen). High-capacity cDNA reverse transcription kits were purchased from Applied Biosystems. RNeasy Mini Kit was purchased from Qiagen. The primers were supplied from Biotnt (Shanghai).
Plasmid DNA encoding GFP and mouse-VEGF-A siRNA expression vector was constructed from pGPU6/GFP/Neo vector (Bioroot Biology, Shanghai, China) according to a previous report. The targeted VEGF-A sequences were 5′- CGATGAAGCCCTGGAGTGC -3′. And the plasmid was amplified using EndoFreeTM Plasmid Maxi (Qiagen). High-capacity cDNA reverse transcription kits were purchased from Applied Biosystems. RNeasy Mini Kit was purchased from Qiagen. The primers were supplied from Biotnt (Shanghai).
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