Brunello knockout pooled library

Manufactured by Addgene

The Brunello knockout pooled library is a powerful tool for gene knockouts in cell lines. It consists of a pool of lentiviral single-guide RNA (sgRNA) targeting thousands of human genes. The library can be used to perform large-scale functional genomic screens to identify genes involved in specific biological processes or responses.

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Lab products found in correlation

Lentiguide puro, by Addgene (3 mentions) Lenticrisprv2 puro, by Addgene (3 mentions) Pfugw efsp cas9 p2a zeo pawp30, by Addgene (3 mentions) Pspax2, by Addgene (3 mentions) Plenti cmv gfp puro 658 5, by Addgene (2 mentions) Phage mt mkeima, by Addgene (2 mentions) Mito bfp, by Addgene (2 mentions) Pgw1 mcherry egfp pim, by Addgene (2 mentions) Plko 1 hygro, by Addgene (2 mentions) Pcmv vsv g, by Addgene (2 mentions) Plenti cmv gfp puro, by Addgene (1 mentions)

3 protocols using brunello knockout pooled library

Shared Plasmid Resource Protocols

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The Brunello knockout pooled library was a gift from David Root and John Doench (Addgene #73178). psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260). pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid #8454). lentiCRISPRv2puro was a gift from Brett Stringer (Addgene plasmid #98290). lentiGuide-puro was a gift from Feng Zhang (Addgene plasmid #52963). pFUGW-EFSp-Cas9-P2A-Zeo (pAWp30) was a gift from Timothy Lu (Addgene plasmid #73857). pLenti CMV GFP Puro (658-5) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid #17448). mitoBFP was a gift from Gia Voeltz (Addgene # 49151). pGW1-mCherry-EGFP-PIM was a gift from Lukas Kapitein (Addgene plasmid #111759). pHAGE-mt-mKeima was a gift from Richard Youle (Addgene plasmid #131626). pLKO.1 hygro was a gift from Bob Weinberg (Addgene plasmid # 24150) Other vectors generated during this study are available upon request.

Delgado J.M., Shepard L.W., Lamson S.W., Liu S.L, & Shoemaker C.J. (2023). The ER membrane protein complex restricts mitophagy by controlling BNIP3 turnover. The EMBO Journal, 43(1), 32-60.

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Plasmid Library and Vector Repository

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Brunello knockout pooled library was a gift from David Root and John Doench (Addgene #73178). psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260). pCMV‐VSV‐G was a gift from Bob Weinberg (Addgene plasmid #8454). lentiCRISPRv2 puro was a gift from Brett Stringer (Addgene plasmid #98290). lentiGuide‐puro was a gift from Feng Zhang (Addgene plasmid #52963). pFUGW‐EFSp‐Cas9‐P2A‐Zeo (pAWp30) was a gift from Timothy Lu (Addgene plasmid #73857). pLenti CMV GFP Puro (658‐5) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid #17448). pHAGE‐myc‐AP2‐TAX1BP1 was a gift from Christian Behrends). pCMV‐hATG7WT and pCMV‐hATG7CS were gifts from Eiki Kominami & Isei Tanida (Addgene plasmids #87867 and #87868). Other vectors generated in the course of this study are available upon request.

Ohnstad A.E., Delgado J.M., North B.J., Nasa I., Kettenbach A.N., Schultz S.W, & Shoemaker C.J. (2020). Receptor‐mediated clustering of FIP200 bypasses the role of LC3 lipidation in autophagy. The EMBO Journal, 39(24), e104948.

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Plasmids for CRISPR and Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Brunello knockout pooled library was a gift from David Rootand John Doench (Addgene #73178). psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260). pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid #8454). lentiCRISPRv2puro was a gift from Brett Stringer (Addgene plasmid #98290). lentiGuide-puro was a gift from Feng Zhang (Addgene plasmid #52963). pFUGW-EFSp-Cas9-P2A-Zeo (pAWp30) was a gift from Timothy Lu (Addgene plasmid #73857). pLenti CMV GFP Puro (658–5) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid #17448). mito-BFP was a gift from Gia Voeltz (Addgene # 49151). pGW1-mCherry-EGFP-PIM was a gift from Lukas Kapitein (Addgene plasmid #111759). pHAGE-mt-mKeima was a gift from Richard Youle (Addgene plasmid #131626). pLKO.1 hygro was a gift from Bob Weinberg (Addgene plasmid # 24150) Other vectors generated during this study are available upon request.

Delgado J.M., Wallace Shepard L., Lamson S.W., Liu S.L, & Shoemaker C.J. (2023). The ER membrane protein complex governs lysosomal turnover of a mitochondrial tail-anchored protein, BNIP3, to restrict mitophagy. bioRxiv.

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