Fc block anti mouse cd16 32 mab

Manufactured by BD

Sourced in United States

Fc Block (anti-mouse CD16/32 mAb) is a laboratory reagent that binds to the Fc receptors CD16 and CD32 on mouse cells. This effectively blocks the non-specific binding of antibodies to these receptors, reducing background signal in immunoassays and flow cytometry experiments.

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Lab products found in correlation

Cd11b clone m1 70, by Thermo Fisher Scientific (2 mentions) Cd3 clone 145 2c11, by Thermo Fisher Scientific (2 mentions) Cd8 clone 53 6, by Thermo Fisher Scientific (2 mentions) Fixation permeabilization buffer, by Thermo Fisher Scientific (2 mentions) Pe anti mouse cd3 clone 17a2, by BioLegend (1 mentions) Pe cy7 anti mouse cd4 clone rm4 5, by BioLegend (1 mentions) Pe anti mouse gr 1 clone rb6 8c5, by BioLegend (1 mentions) Facs celesta flow cytometer, by FlowJo (1 mentions) Collagenase type 1, by Merck Group (1 mentions) Dnase, by Merck Group (1 mentions) Cd45 clone 30 f11, by Thermo Fisher Scientific (1 mentions) Ly6c clone hk1.4, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Solarbio (1 mentions) Lsrfortessa, by BD (1 mentions) Interferon γ ifn γ, by BD (1 mentions) Facsverse, by BD (1 mentions) Granzyme b, by BD (1 mentions) Granzyme b clone gb11, by Thermo Fisher Scientific (1 mentions) Fortessa flow cytometer, by BD (1 mentions)

Spelling variants of this product

Mouse BD Fc Block (226 citations) Anti-CD16/32 (78 citations) Anti-mouse CD16/32 (45 citations) Fc block (anti-CD16/32, (33 citations) CD16/32 (Fc block; (21 citations) Anti-mouse CD16/32 (Fc Block; (17 citations) Anti-mouse Fc Block (11 citations) Anti-CD16/32 mAb (4 citations) Mouse Fc block (anti-mouse CD16/32) (2 citations) CD16/32 Mouse BD Fc Block (2 citations)

5 protocols using fc block anti mouse cd16 32 mab

Multiparametric Flow Cytometry of Tumor-Infiltrating Immune Cells

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Single-cell suspensions of tumor-burdened lungs were re-suspended in FACS buffer (PBS, 1%BSA), blocked by non-specific staining with Fc block (anti-mouse CD16/32 mAb; BD Biosciences), The following antibodies were acquired from Biolegend and used in the flow cytometry analysis: BV605 anti-mouse CD45 (clone 30-F11), PE anti-mouse CD3 (clone 17A2), PE/Cy7 anti-mouse CD4 (clone RM4-5), BV711 anti-mouse CD8α (clone 53 − 6.7), BV421 anti-mouse Ly-6 C (clone HK1.4), PE anti-mouse Gr-1 (clone RB6-8C5), PE/Cy5 anti-mouse CD11b (clone M1/70), PE/Cy7 anti-mouse Ly-6G (clone 1A8), BV711 anti-mouse IFN-γ (clone XMG1.2), BV421 anti-mouse CD8α (clone 53 − 6.7), PE/Cy5 anti-mouse CD3 (clone 17A2). Flow cytometry data were acquired on a FACS Celesta flow cytometer and data was analysed with FlowJo V10.

Liang M., Sun Z., Chen X., Wang L., Wang H., Qin L., Zhao W, & Geng B. (2023). E3 ligase TRIM28 promotes anti-PD-1 resistance in non-small cell lung cancer by enhancing the recruitment of myeloid-derived suppressor cells. Journal of Experimental & Clinical Cancer Research : CR, 42, 275.

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Multiparameter Flow Cytometry of Immune Cells

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Bone marrow cells were flushed from the femurs and tibias with PBS with a syringe. The spleen samples were processed through mechanical dissociation, and tumour tissues were processed into single-cell suspensions by dissociating the tissues enzymatically for 1 h with 1 mg/ml type I collagenase (Sigma-Aldrich) in the presence of 50 units/mL DNase (Sigma-Aldrich). The cells were lysed with red blood cell lysis buffer and filtered with a 100 μm membrane, further washed with 1% BSA in PBS and blocked by non-specific staining with Fc Block (anti-mouse CD16/32 mAb; BD Biosciences). The samples were then stained with fluorescence-conjugated antibodies against the surface markers CD45 (clone 30-F11, eBioscience), CD11b (clone M1/70, eBioscience), Ly6C (clone HK1.4, eBioscience), Ly6G (clone 1A8-Ly6g, eBioscience), CD3 (clone 145-2C11, eBioscience) and CD8 (clone 53–6.7, eBioscience) and detected using flow cytometry (LSR BD Fortessa).

Gao W., Zhang X., Yang W., Dou D., Zhang H., Tang Y., Zhong W., Meng J., Bai Y., Liu Y., Yang L., Chen S., Liu H., Yang C, & Sun T. (2019). Prim-O-glucosylcimifugin enhances the antitumour effect of PD-1 inhibition by targeting myeloid-derived suppressor cells. Journal for Immunotherapy of Cancer, 7, 231.

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Multicolor Flow Cytometry Profiling

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The samples were washed with 1% bovine serum albumin (BSA) (Solarbio, Beijing, China) in PBS, blocked by non-specific staining with Fc block (anti-mouse CD16/32 mAb; BD Biosciences, Franklin Lakes, U.S.A), and stained with fluorescence-conjugated antibodies against surface markers CD45 (clone 30-F11), CD11b (clone M1/70), Gr-1 (clone 11-5931-81), Ly6G (clone 1A8-Ly6g), Ly6C(clone HK1.4), CD3 (clone 145-2C11), and CD8 (clone 53-6.7) (eBioscience, California, U.S.A) and detected by flow cytometry (BD LSRFortessa, Franklin Lakes, U.S.A).

Li Y., Qiao K., Zhang X., Liu H., Zhang H., Li Z., Liu Y, & Sun T. (2021). Targeting myeloid-derived suppressor cells to attenuate vasculogenic mimicry and synergistically enhance the anti-tumor effect of PD-1 inhibitor. iScience, 24(12), 103392.

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Tumor Immune Cell Characterization

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Tumors were digested with collagenase and hyaluronidase for 1 h at 37°C. After lysising of red blood cell, the dissociated cells were incubated on ice for 10 min, and then spun down at 300 g, 4°C for 7 min, cells from these tumors were either used for flow cytometry analysis or further processed and used for functional analyses. Tumor cell suspensions, were washed, blocked with Fc Block (anti-mouse CD16/32 mAb; BD Biosciences) at 4°C on ice for 15 min, and stained with fluorescence conjugated antibodies against surface markers CD49b, CD3E, CD8a, and CD25. These antibodies were purchased from BioLegend, eBioscience, or BD Biosciences. Cells were then fixed in Fixation/Permeabilization buffer (eBioscience) and stained with antibodies against intracellular proteins, including FoxP3 (BioLegend), granzyme B and interferon-γ (IFN-γ) (BD Pharmingen). Stained cells and isotype-control-stained cells, were assayed using a BD FACSVerse (BD Biosciences, United States). Data analysis was performed using the FlowJo (Tree Star) software.

Liu J., Jiang M., Li Z., Zhang X., Li X., Hao Y., Su X., Zhu J., Zheng C., Xiao W, & Wang Y. (2018). A Novel Systems Pharmacology Method to Investigate Molecular Mechanisms of Scutellaria barbata D. Don for Non-small Cell Lung Cancer. Frontiers in Pharmacology, 9, 1473.

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Multiparameter Flow Cytometry of Immune Cells

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Splenocytes, tumor cell suspensions, and peripheral blood cells were washed, blocked with Fc Block (anti-mouse CD16/32 mAb; BD Biosciences) at 4°C for 15 minutes, and stained with fluorescence conjugated antibodies against surface markers CD45(clone 30-F11), CD11b (clone M1/70), CD11c, Gr1 (clone RB6-8C5), Ly6C (clone AL-21), Ly6G (clone 1A8), F480 (clone BM8), I-Ab, I-Ad, CD8a (clone 53-6.7), CD4 (clone RM4-5) antibodies purchased from BioLegend, eBioscience or BD Biosciences. Cells were then fixed in Fixation/Permeabilization buffer (eBioscience) and stained with antibodies against intracellular proteins, including FoxP3 (NRRF-30) and Granzyme B (clone GB11). The antibodies were purchased from BD Biosciences, Biolegend, and R&D Systems and used in staining. Lineage antibody cocktail was purchased from eBioscience. Anti-mouse CCR2 antibody was purchased from R&D Systems, Inc. Stained cells and isotype-control-stained cells, were assayed using a LSRII, LSR4 or Fortessa flow cytometer (BD Biosciences). Data analysis was performed using the FlowJo (FlowJo LLC, Tree Star) and/or ModFit LT 4.1 software.

Orillion A., Hashimoto A., Damayanti N., Shen L., Adelaiye-Ogala R., Arisa S., Chintala S., Ordentlich P., Kao C., Elzey B., Gabrilovich D, & Pili R. (2017). Entinostat neutralizes myeloid derived suppressor cells and enhances the antitumor effect of PD-1 inhibition in murine models of lung and renal cell carcinoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(17), 5187-5201.

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