Htopo 2

Manufactured by TopoGEN

Sourced in United States

The HTopo II is a laboratory instrument designed for the analysis and manipulation of DNA topological structures. It provides researchers with the capability to visualize and quantify the supercoiled, relaxed, and knotted forms of DNA molecules.

Automatically generated - may contain errors

Lab products found in correlation

Htopo 1, by TopoGEN (2 mentions) Compounds ranhc 5 and 6, by TopoGEN (1 mentions) Kinetoplast dna kdna, by TopoGEN (1 mentions)

3 protocols using htopo 2

Topoisomerase I and II Inhibition Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

hTopoI relaxation assays were done using the supercoiled pHOT1 as substrate, with the recombinant hTopoI (TopoGEN, Port Orange, FL, USA) and compounds RANHC-V and -VI or DMSO (CTRL) following the manufacturer’s protocol (TopoGEN, Port Orange, FL, USA), with some changes [47 (link)].
Similarly, hTopoII decatenation assays were carried out incubating the kinetoplast DNA (kDNA) substrate with the hTopoII (TopoGEN, Port Orange, FL) and compounds RANHC-V and -VI or DMSO (CTRL) following the manufacturer’s procedures (TopoGEN, Port Orange, FL, USA), with some changes [47 (link)].

Ceramella J., Troiano R., Iacopetta D., Mariconda A., Pellegrino M., Catalano A., Saturnino C., Aquaro S., Sinicropi M.S, & Longo P. (2023). Synthesis of Novel N-Heterocyclic Carbene-Ruthenium (II) Complexes, “Precious” Tools with Antibacterial, Anticancer and Antioxidant Properties. Antibiotics, 12(4), 693.

+ Open protocol

+ Expand

Topoisomerase Relaxation and Decatenation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

hTopo I relaxation assays were performed by incubating the substrate (supercoiled pHOT1) with the recombinant hTopo I (TopoGEN, Port Orange, FL, USA) and compounds, as indicated in the supplier protocol (TopoGEN, Port Orange, FL, USA), with some revisions [80 (link)]. hTopo II decatenation assays were conducted by incubating the kinetoplast DNA substrate (kDNA) with the hTopo II (TopoGEN, Port Orange, FL) and studied compounds, as indicated in the supplier procedures (TopoGEN, Port Orange, FL, USA), with some changes as in [80 (link)].

Sinicropi M.S., Ceramella J., Vanelle P., Iacopetta D., Rosano C., Khoumeri O., Abdelmohsen S., Abdelhady W, & El-Kashef H. (2023). Novel Thiazolidine-2,4-dione-trimethoxybenzene-thiazole Hybrids as Human Topoisomerases Inhibitors. Pharmaceuticals, 16(7), 946.

+ Open protocol

+ Expand

Topoisomerase II Decatenation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

hTopo II decatenation assays were performed in a final volume of 20 mL, as reported by Iacopetta et al. [37 (link),78 (link)], using 0.2 mg of kinetoplast DNA (kDNA) (topoGEN, Port Orange, FL, USA), tested compounds and 3 units of hTopo II (topoGEN, Port Orange, FL, USA). The reaction products were loaded in an agarose gel with EB. At the end, the gel was washed with distilled water and analyzed using a UV transilluminator. The experiment was repeated three times.

Iacopetta D., Rosano C., Sirignano M., Mariconda A., Ceramella J., Ponassi M., Saturnino C., Sinicropi M.S, & Longo P. (2020). Is the Way to Fight Cancer Paved with Gold? Metal-Based Carbene Complexes with Multiple and Fascinating Biological Features. Pharmaceuticals, 13(5), 91.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!