Sc7977

Manufactured by Santa Cruz Biotechnology

Sourced in United Kingdom

Sc7977 is a high-quality laboratory equipment product offered by Santa Cruz Biotechnology. It is designed to perform essential functions in scientific research and analysis. The core function of this product is to provide reliable and precise measurements or data collection, as required by researchers and laboratory professionals. No further details or interpretations about the intended use of this product can be provided in this unbiased and factual description.

Automatically generated - may contain errors

Lab products found in correlation

Ab8226, by Abcam (1 mentions) V9131, by Merck Group (1 mentions) Ar441, by Agilent Technologies (1 mentions) Ecl system, by GE Healthcare (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Anti lc3b, by Merck Group (1 mentions) Pierce protease and phosphatase inhibitor mini tablet, by Thermo Fisher Scientific (1 mentions) Anti mcu, by Cell Signaling Technology (1 mentions) Sc 136178, by Santa Cruz Biotechnology (1 mentions) Anti iκb α sc 1643, by Santa Cruz Biotechnology (1 mentions)

2 protocols using sc7977

Western Blot Protein Detection Protocol

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Equal amounts of total protein were separated by SDS‐PAGE, transferred onto PVDF membrane (Millipore, Watford, UK) and probed using primary antibodies: mouse anti‐p23 (1:3000, ab2814, Abcam), rabbit anti‐AR (AR441, 1:1000, Dako), rabbit anti‐HSP90 (sc7977, 1:500, SantaCruz), mouse anti‐β‐actin (ab8226, 1:8000, Abcam), mouse anti‐αtubulin (DM1A, 1:1000, Sigma) and mouse anti‐vinculin (V9131, 1:1000, Sigma). Proteins were detected using horseradish peroxidise‐conjugated anti‐mouse or anti‐rabbit secondary antibodies (1:2000, DAKO) and visualised using the ECL system (GE Healthcare).

Querol Cano L., Lavery D.N., Sin S., Spanjaard E., Brooke G.N., Tilman J.D., Abroaf A., Gaughan L., Robson C.N., Heer R., Mauri F., de Rooij J., Driouch K, & Bevan C.L. (2014). The co‐chaperone p23 promotes prostate cancer motility and metastasis. Molecular Oncology, 9(1), 295-308.

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Analysis of Cardiac Protein Expression

Cited 1 time

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Isolated heart tissue was homogenized in radioimmune precipitation assay buffer containing protease and phosphatase inhibitors (Pierce Protease and Phosphatase Inhibitor Mini Tablets, Thermo Scientific). 30–50 μg of total protein extract was applied to SDS-PAGE and transferred onto PVDF membranes. Antibodies used for this study include anti-IκBα (sc-1643, Santa Cruz), anti-phosphoIκBα at Ser-32 (sc-7977, Santa Cruz), anti-LC3B (Sigma L7543), anti-MCU (Cell Signaling D2Z3B), anti-MICU1[21 (link)], and anti-ATP5A (Santa Cruz sc-136178).

Kokkinaki D., Hoffman M., Kalliora C., Kyriazis I.D., Maning J., Lucchese A.M., Shanmughapriya S., Tomar D., Park J.Y., Wang H., Yang X.F., Madesh M., Lymperopoulos A., Koch W.J., Christofidou-Solomidou M, & Drosatos K. (2019). Chemically synthesized Secoisolariciresinol diglucoside (LGM2605) improves mitochondrial function in cardiac myocytes and alleviates septic cardiomyopathy. Journal of molecular and cellular cardiology, 127, 232-245.

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