Log In Sign up
The largest database of trusted experimental protocols

Elisa grade collagen

Manufactured by Chondrex
Visit Supplier
Sourced in United States

ELISA-grade collagen is a high-purity collagen product designed for use in enzyme-linked immunosorbent assay (ELISA) applications. It is manufactured to meet the stringent quality standards required for ELISA testing.

Automatically generated - may contain errors

Lab products found in correlation

O phenylenediamine dihydrochloride substrate, by Merck Group (2 mentions)

2 protocols using elisa grade collagen

1

Quantification of Cytokines, Chemokines, and Anti-Collagen Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytokines, chemokines and anti-collagen antibody (Ab) levels were quantified by Luminex (Life Technologies) or ELISA using appropriately diluted sera or culture supernatants. Reagents for the quantification of mouse chemokines, IFNγ, IL-17 and tumor necrosis factor-α were from Biosource (Life Technologies), and assays were performed according to the manufacturer's instructions. Anti-collagen Ab titers of individual sera were evaluated using ELISA-grade collagen (Chondrex) and detected with horseradish peroxidase-conjugated anti-mouse IgG1 or IgG2a (Southern Biotech, Birmingham, AL, USA). Total IgG was determined using an unlabeled anti-mouse IgG capture Ab and detected with horseradish peroxidase-conjugated anti-mouse IgG1 or IgG2a. Antibody ELISAs were developed using o-phenylenediamine dihydrochloride substrate (Sigma-Aldrich).
Hansell C.A., MacLellan L.M., Oldham R.S., Doonan J., Chapple K.J., Anderson E.J., Linington C., McInnes I.B., Nibbs R.J, & Goodyear C.S. (2014). The atypical chemokine receptor ACKR2 suppresses Th17 responses to protein autoantigens. Immunology and Cell Biology, 93(2), 167-176.
+ Open protocol
+ Expand
2

Quantifying Immune Responses in Murine Models

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytokines, chemokines and anti-collagen Ab levels were quantified by Luminex or ELISA using appropriately diluted sera or culture supernatants. Reagents for the quantification of mouse chemokines, IFNγ, IL-17 and TNFα were from Biosource, and assays were performed according to the manufacturer’s instructions. Anti-collagen Ab titers of individual sera were evaluated using ELISA-grade collagen (Chondrex) and detected with HRP-conjugated anti-mouse IgG1 or IgG2a (Southern Biotech). Total IgG was determined using an unlabeled anti-mouse IgG capture antibody and detected with HRP-conjugated anti-mouse IgG1 or IgG2a. Antibody ELISAs were developed using o-phenylenediamine dihydrochloride substrate (Sigma).
Hansell C.A., MacLellan L.M., Oldham R.S., Doonan J., Chapple K.J., Anderson E.J., Linington C., McInnes I.B., Nibbs R.J, & Goodyear C.S. (2014). The atypical chemokine receptor ACKR2 suppresses Th17 responses to protein autoantigens. Immunology and Cell Biology, 93(2), 167-176.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!