Wogonin

Dulbecco’s modified Eagle’s medium (DMEM) and other reagents for cell culture were purchased from HyClone Laboratories (Logan, UT, USA). Ham’s F12 Medium was purchased from Lonza (Walkersville, MD, USA). Pronase and Collagenase were from Roche Diagnostics (Indianapolis, IN, USA). Wogonin was purchased from Extrasynthese (Genay Cedex, France). Recombinant human IL-1β, anti-COX-2 antibody and PGE₂ and IL-6 ELISA kit were from R&D Systems (St Paul, MN, USA). Dichlorodihydrofluorescein diacetate (H₂DCF-DA), fluorescein diacetate (FDA), dihydro-rhodamine (DHR123), propidium iodide (PI) were procured from Sigma Aldrich Chemicals Inc. (St. Louis, MO, USA). Antibodies specific for p-ERK1/2, ERK1/2, p-JNK, JNK, p-P38, P38, Nrf2, p-PI3K, p-AKT, p-GSK3β and Iκβα were purchased from Cell Signaling Technology (Beverly, MA). Antibodies specific for β-Actin, MMP-13, MMP-3, MMP-9, IL-6, iNOS, and COL2A1 were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Appropriate horseradish peroxidase conjugated secondary antibodies were from Cell Signaling Technology (#7074) or Santa Cruz Biotechnology (sc-2020).

Dulbecco’s modified Eagle’s medium (DMEM) was purchased from HyClone Laboratories (Logan, UT, USA). Ham’s F12 Medium was purchased from Lonza (Walkersville, MD, USA). Pronase and Collagenase were from Roche Diagnostics (Indianapolis, IN, USA). Wogonin was purchased from Extrasynthese (Genay Cedex, France). Recombinant human IL-1β and FlowTACS Flow Cytometry Apoptosis Detection Kit were from R&D Systems (St Paul, MN, USA). Thiazolyl blue tetrazolium bromide (MTT), and Propidium Iodide (PI) were from Sigma Aldrich Chemicals Inc. (St. Louis, MO, USA). Antibodies specific for Caspase-3, Caspase-8 and Caspase-9 were purchased from Cell Signaling Technology (Beverly, MA) and anti-β-actin antibody, Bcl2, Bcl-xl was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Appropriate horseradish peroxidase conjugated secondary antibodies were from Cell Signaling Technology (#7074) or Santa Cruz Biotechnology (sc-2020).