Spinning disk confocal imaging was performed with a Quorum spinning disk confocal head on a Zeiss Axiovert 200M microscope, equipped with a 63× NA 1.4 oil objective and 25× NA 0.8 multi-immersion objective, a 1.5× tube lens, and a back-thinned EM-CCD camera (C9100–13, Hamamatsu). Acquisition settings were controlled using Volocity software and were acquired at equal laser and exposure settings within experiments across compared conditions. Lightning microscopy was performed on a Leica SP8 Lightning Confocal DMI6000 microscope utilizing a 100× 1.4 NA (O, STED) objective and two HyD detectors. Acquisitions were driven by a Leica motorized XY stage and Leica SuperZ Galvo with Adaptive Focus Control. Acquisition settings were controlled using the Leica LAS, Lightning Module software, with matched settings within experiments.
Superz galvo
Manufactured by Leica
The SuperZ Galvo is a high-precision scanning mirror system designed for applications that require fast and accurate laser beam positioning. It features a low-inertia galvanometer scanner with a wide angular range and high positioning speed. The SuperZ Galvo is a core component in various laboratory equipment and scientific instrumentation.
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2 protocols using superz galvo
1
Spinning Disk and Lightning Confocal Imaging
2
Spinning Disk and Lightning Confocal Imaging
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Spinning disk confocal imaging was performed with a Quorum spinning disk confocal head on a Zeiss Axiovert 200M microscope, equipped with a 63× NA 1.4 oil objective and 25× NA 0.8 multi-immersion objective, a 1.5× tube lens, and a back-thinned EM-CCD camera (C9100–13, Hamamatsu). Acquisition settings were controlled using Volocity software and were acquired at equal laser and exposure settings within experiments across compared conditions. Lightning microscopy was performed on a Leica SP8 Lightning Confocal DMI6000 microscope utilizing a 100× 1.4 NA (O, STED) objective and two HyD detectors. Acquisitions were driven by a Leica motorized XY stage and Leica SuperZ Galvo with Adaptive Focus Control. Acquisition settings were controlled using the Leica LAS, Lightning Module software, with matched settings within experiments.
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