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3 protocols using tata binding protein antibody

1

Investigating STAT3 Signaling Pathway

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Antibodies to STAT3 and phosphoSTAT3 were obtained from Cell Signaling technologies. Antibodies to JunB and RNA polymerase II as well as rabbit immunoglobulin G (IgG) were obtained from Santacruz. The TATA binding protein (TBP) antibody was obtained from Abcam. The fibronectin antibody was obtained from BD Transduction Laboratories. SD1029 and AG490 were obtained from Calbiochem. All DNA primers were obtained from Integrated DNA Technologies. TGFβ, IL6, and IL6Rα were obtained from R&D Systems.
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2

Molecular Mechanisms of ER Stress Regulation

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Human thrombin was obtained from Enzyme Research Laboratories (South Bend, IN). Tunicamycin was purchased from Sigma. Polyclonal antibodies to ICAM-1, VCAM-1, RelA/p65, β-actin, IκBα, and Lamin B were from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies to phospho-eIF2α, eIF2α, BiP/GRP78, ATF-4, phospho-AKT, phospho-IKKβ, IKKβ, phospho-(Ser536)-RelA/p65, were obtained from Cell Signaling (Beverly, MA). Tata Binding Protein (TBP) antibody was purchased from Abcam. RelA/p65 transcription factor assay kit was purchased from Cayman Chemical (Ann Arbor, MI) and plasmid maxi kit was from QIAGEN Inc. (Valencia, CA). Protein assay kit and nitrocellulose membrane were from Bio-Rad. Alexa Fluor 488-phalloidin was purchased from Invitrogen. Expression vector encoding Wild type BiP and dominant negative BiP were from Addgene. All other materials were from VWR Scientific Products Corporation (Gaithersburg, MD) and Fisher Scientific (Pittsburgh, PA). SubAB and its non-toxic derivative SubAA272B were purified as previously described [37] (link).
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3

Evaluating Inflammatory Signaling Pathways

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Human thrombin was obtained from Enzyme Research Laboratories (South Bend, IN). Polyclonal antibodies to ICAM-1, VCAM-1, RelA/p65, β-actin, and IκBα were from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-(Ser536)-RelA/p65, was obtained from Cell Signaling (Beverly, MA). Importin α, importin β and TATA Binding Protein (TBP) antibody was purchased from Abcam (Cambridge, MA). NF-κB (p65) transcription factor assay kit was purchased from Cayman Chemical (Ann Arbor, MI). All other materials including Alexa Fluor 488-phalloidin were from Thermo Fisher Scientific (Waltham, MA).
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