Macs quantify version 2

Manufactured by Miltenyi Biotec

The MACS Quantify version 2.3 software is a data analysis tool designed for use with Miltenyi Biotec's flow cytometry instruments. The software provides users with the ability to analyze and quantify data from cellular samples.

Automatically generated - may contain errors

Lab products found in correlation

Annexin 5 fitc propidium iodide detection kit, by Merck Group (2 mentions) Facscalibur flow cytometer, by BD (1 mentions) Cellquest pro software, by BD (1 mentions) Mitoscreen kit, by BD (1 mentions) Macsquant analyzer, by Miltenyi Biotec (1 mentions)

2 protocols using macs quantify version 2

Evaluating Apoptosis and Mitochondrial Potential

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the evaluation of JC-1 staining in control and apoptotic cells, the instruction manual of flow cytometry mitochondrial membrane potential detection kit (BD™ MitoScreen Kit) was followed. Results were analyzed by FACS Calibur flow cytometer and the Cell Quest Pro software (BD Biosciences).
Additionally, the number of cells undergoing apoptosis (early or late) was quantified using the Annexin V-FITC/propidium iodide (PI) detection kit (Merck Millipore), following the manufacturer's instructions. Data were analyzed by MACSQuant analyzer flow cytometry using the MACS Quantify version 2.3 software (Miltenyi Biotec).

Falgàs A., Pallarès V., Serna N., Sánchez-García L., Sierra J., Gallardo A., Alba-Castellón L., Álamo P., Unzueta U., Villaverde A., Vázquez E., Mangues R, & Casanova I. (2020). Selective delivery of T22-PE24-H6 to CXCR4+ diffuse large B-cell lymphoma cells leads to wide therapeutic index in a disseminated mouse model. Theranostics, 10(12), 5169-5180.

+ Open protocol

+ Expand

Annexin V-FITC/PI Assay for T22-Cytotoxin-Induced Cell Death

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell death induced by T22-PE24-H6 and T22-DITOX-H6 was further assessed using the Annexin V-FITC / propidium iodide (PI) detection kit (Merck Millipore) following manufacturer’s instructions. 74B-CXCR4⁺ cells were cultured in 6-well plates (25,000 cells/well) and exposed to 50 nM of either of the two nanotoxins for different times (15 h, 24 h, and 48 h). Cells were analyzed by MACSQuant analyzer flow cytometry with the MACS Quantify version 2.3 software (Miltenyi Biotech). The experiment was performed in triplicate.

Rioja-Blanco E., Arroyo-Solera I., Álamo P., Casanova I., Gallardo A., Unzueta U., Serna N., Sánchez-García L., Quer M., Villaverde A., Vázquez E., León X., Alba-Castellón L, & Mangues R. (2022). CXCR4-targeted nanotoxins induce GSDME-dependent pyroptosis in head and neck squamous cell carcinoma. Journal of Experimental & Clinical Cancer Research : CR, 41, 49.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!