Bis tris hcl

The human neuroblastoma cell line SH-SY5Y was obtained from National Centre for Cell Sciences, Pune, India. Low glucose Dulbecco’s modified Eagle’s medium, minimal essential medium modified for suspension culture (S-MEM), fetal bovine serum, Gentamicin, tetramethylrhodamine, methyl ester (TMRM), were procured from GIBCO, Invitrogen Corporation (CA, USA). Lymphocyte separation medium (LSM), polyethylene glycol 1000, manitol, were purchased from MP Biomedicals (France). PicoGreen®, MitoSOX™ were purchased from Molecular probes (OR, USA). Potassium chloride, KH₂PO₄, NaCl and Na₂HPO₄.2H₂O, magnesium sulfate, lead nitrate, sucrose, were procured from Sisco Research laboratories (India). Amphotericin B, pyruvate, uridine, ethidium bromide, TRI reagent, 6-aminocaproic acid, Bis-Tris HCl, N-Dodecyl β-D-Maltoside, bis acrylamide, ATP, EGTA were purchased from Sigma (MO, USA). Coomassie Brilliant Blue G-250 and R-250 dyes were purchased from BioRad (CA, USA) and acrylamide was purchased from Merck (Darmstadt, Germany).