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Pannoramic 250 microscope

Manufactured by 3DHISTECH
Sourced in Hungary

The Pannoramic‐250 is a high-performance digital slide scanner designed for whole slide imaging. It captures high-resolution images of microscope slides with a maximum scan area of 24 mm x 76 mm. The scanner utilizes a unique optical design and advanced image processing algorithms to produce detailed, high-quality digital slides suitable for a variety of applications.

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3 protocols using pannoramic 250 microscope

1

Histological Tissue Processing and Imaging

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Skin was fixed in 10% neutral buffered formalin, embedded in paraffin and cut to 5 μm. Haematoxylin and eosin staining was performed by Shandon Varistain V24‐4. Image acquisition used 3D‐Histech Pannoramic‐250 microscope slide‐scanner and Case Viewer software (3D‐Histech).
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2

Ovarian Histopathology and Follicle Counting

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Ovarian histopathological examination and cystic follicle count were performed based on a previous study (Qi et al., 2019 (link)). Ovary tissues were quickly collected and fixed in 4% paraformaldehyde. Subsequently, a series of operations, such as dehydration, paraffin embedding, and segmentation, were performed. The sections were stained with hematoxylin and eosin for histopathological examination using a Pannoramic250 microscope (3DHISTECH Ltd., Budapest, Hungary).
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3

Histological Analysis of Ear Tissue

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Ear tissue was fixed in 10% neutral buffered formalin (Sigma Aldrich, Gillingham, UK), then embedded in paraffin and cut to 5 μm. Hematoxylin and eosin staining was carried out using an automated Shandon Varistain V24-4 (Waltham, MA, USA). Images were acquired using a 3D HISTECH Pannoramic-250 microscope slide-scanner (3D HISTECH, Budapest, Hungary). Snapshots were taken with Case Viewer software (3D HISTECH, Budapest, Hungary).
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