Primary human umbilical vein endothelial cells (HUVECs; C-12200 PromoCell GmbH, Heidelberg, DEU) and primary human umbilical artery smooth muscle cells (HUASMCs; C-12500 PromoCell GmbH, Heidelberg, DEU), were used to assess vascular cell adhesion onto the PCL film samples. HUVECs were cultured in endothelial cell growth medium (C-22011 PromoCell GmbH, Heidelberg, DEU ) supplemented with 1% (v/v) penicillin-streptomycin, 2% (v/v) fetal bovine serum, 0.4% (v/v) endothelial cell growth supplement, 0.1 ng/ml EGF, 1 ng/ml bFGF, 90 μg/ml heparin, and 1 μg/ml hydrocortisone. HUASMCs were cultured in smooth muscle cell growth medium (C-22062 PromoCell GmbH, Heidelberg, DEU) supplemented with 1% (v/v) antibiotic-antimycotic, 5% (v/v) fetal bovine serum, 0.5 ng/ml EGF, 2 ng/ml bFGF, and 5 μg/ml insulin. All cells were incubated at 37 °C, 5% CO2 and grown to 80% confluency before use with experiments. Only passages between 3 and 6 were used for both cell lines.
C 12500
Manufactured by PromoCell
Sourced in Germany
The C-12500 is a laboratory equipment piece designed for general-purpose applications. It features a compact and durable construction. The core function of the C-12500 is to provide a reliable and consistent performance for various laboratory tasks.
Automatically generated - may contain errors
Lab products found in correlation
C 22062, by PromoCell (1 mentions)
C 22011, by PromoCell (1 mentions)
C 12200, by PromoCell (1 mentions)
Smooth muscle cell growth medium, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
C 12203, by PromoCell (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Human umbilical vein endothelial cells (huvec), by PromoCell (1 mentions)
Huasmc, by PromoCell (1 mentions)
2 protocols using c 12500
1
Vascular cell adhesion on PCL films
2
Culturing Endothelial, Smooth Muscle, and Pericyte Cells
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Studies were performed using pooled primary human umbilical vein endothelial cells (HUVEC, C-12203), human umbilical artery smooth muscle cells (HUASMC, C-12500) and human pericytes from placenta (HPC, hPC-PL, C-12980) purchased from PromoCell (Heidelberg, Germany). Cells were grown in the corresponding media from PromoCell (Heidelberg, Germany): Endothelial Cell Growth Medium 2 (Ready-to-use, C-22011), Smooth Muscle Cell Growth Medium 2 (Ready-to-use, C-22062) and Pericyte Growth Medium (Ready-to-use, C-28040) with addition of 1% penicillin/streptomycin (Gibco). Cells were routinely cultured under nitrogen atmosphere (7% O2, 5% CO2) and passaged twice a week for a maximum of 15 passages using trypsin (Gibco). BPDE was purchased from BIU Biochemical Institute for Environmental Carcinogens, Prof. Dr. G. Grimmer-Stiftung (Grosshansdorf, Germany). BPDE was dissolved in water-free THF/5% trimethylamine (1 mM) and stored at −80 °C. To avoid hydrolysis of the epoxides, dilutions from the stock solution were always prepared fresh immediately before incubation.
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