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Jsm 5200 scanning

Manufactured by JEOL

The JSM-5200 is a scanning electron microscope (SEM) manufactured by JEOL. It is a versatile instrument designed for high-resolution imaging of a wide range of samples. The JSM-5200 utilizes a thermionic emission electron gun and can achieve a maximum magnification of up to 300,000x. It is equipped with various detectors, including a secondary electron detector and a backscattered electron detector, allowing for the examination of surface topography and composition of the sample.

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2 protocols using jsm 5200 scanning

1

Detailed Microscopic Imaging Protocol

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Specimens were photographed using an Olympus Camedia E-520 camera attached to an Olympus SZX16 stereomicroscope or to the eye piece of an Olympus BH2 transmission microscope, and a JEOL JSM-5200 scanning electron microscope (SEM) at the Zoological Museum of University of Turku, Finland. Digital images were prepared using CombineZP image stacking software. Illustrations of internal genitalia were made after clearing them in a 10% KOH aqueous solution. Lengths of leg segments were measured on the dorsal side. Measurements are provided for leg I only (IV, if missing) and listed as: total length (femur, patella, tibia, metatarsus, tarsus). All measurements are given in millimeters.
Abbreviations not explained in the text: ALE – anterior lateral eye, AME – anterior median eye, PLE – posterior lateral eye, PME – posterior median eye.
Depositories: MHNGMuséum d’histoire naturelle, Genève, Switzerland, MMUEManchester Museum of the University of Manchester, England, ZMMUZoological Museum of Moscow University, Moscow, Russia, ZMUTZoological Museum of University of Turku, Finland, PPC – A.V. Ponomarev’s personal collection, Rostov on Don, Russia.
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2

Stereomicroscope and SEM Imaging of Specimens

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Specimens were photographed using a Canon EOS 7D camera attached to an Olympus SZX16 stereomicroscope, and a JEOL JSM-5200 scanning electron microscope at the Zoological Museum of the University of Turku. Digital images were stacked using CombineZP and edited using CorelDraw graphic design software. Figures of the species were made at different times, resulting in variations in their styles. Lengths of leg segments were measured on the dorsal side. All measurements are given in mm. The distribution map is based on the literature and personal data. Not all localities in Fennoscandia and the Nearctic are shown. The type material will be deposited in the
Zoological Museum of Moscow State University (ZMMU).
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