Isoliquiritigenin

OASCs from controls were seeded at a density of 5 × 10³ cells/well in 96-well plates in 100 µL media. After 24 hours, OASCs were treated with Torin 2 (0.01–5.00 µM) (Selleckchem Catalog No. S2817; Selleckchem, Houston, TX), PX12 (0.5–40.0 µM) (Selleckchem Catalog No. S7947), withaferin A (0.05–10.00 µM) (Abcam CAS number 5119-48-2; Abcam, Cambridge, MA), isoliquiritigenin (0.5–50.0 µM) (Selleckchem Catalog No. S2404), mitoxantrone (0.01–5.00 µM) (Selleckchem Catalog No. S2485), and MLN8054 (0.5–50.0 µM) (Selleckchem Catalog No. S1100) for 72 hours. After treatment, 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA) was performed according to the manufacturer's instructions to measure the mitochondrial metabolism rate as an indicator for proliferation and viability. MTT (5 mg/mL) was added and plates were incubated at 37°C for 4 hours before dimethyl sulfoxide (100 µL) was added to each well. Upon verifying dissolution of the formazan salt, the absorbance of each well was read at a wavelength of 570 nm using a scanning multiwell spectrophotometer. The results of three identically treated, independent wells were averaged to yield corresponding cell viability measurements.