4 nitrophenol

All chemicals and reagents used were of analytical grade. Methanol and acetonitrile were purchased from Merck Co., Inc. (Darmstadt, Germany). Fructose, glucose, sucrose, and maltose standards were obtained from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). 3,5-Dinitrosalicylic acid and indigo carmine were purchased from Shanghai Dibo Biotechnology Co., Ltd. (Shanghai, China). 4-nitrophenol and gallic acid were from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). DPPH and TPTZ were purchased from Sigma-Aldrich (St. Louis, MI, USA). Proline and rutin were obtained from Beijing Bailingwei Technology Co., Ltd. (Beijing, China).

E. coli DH5α for gene cloning were stored in our lab. E. coli BL21, BL21 (DE3), BL21 (DE3) pLysS, BL21 CodonPlus (DE3) were purchased from TransGen Biotechnology (China). E. coli C41, C43 and Rossetta (DE3) were purchased from Biyotime (China). Plasmid pET23a-sfGFP₍₋₁₅₎ was stored in our lab. Luria–Bertani (LB) were prepared as described in the Manual of Molecular Cloning [18 ]. The substrate 4-nitrophenyl palmitate (p-NPP) was purchased from Macklin (USA) and 4-Nitrophenol was purchased from Macklin (USA). Dibutyl phthalate (DBP), bis(2-ethylhexyl) phthalate (DEHP), dicyclohexyl phthalate (DCHP) were purchased from ANPEL-Trace Standard Technical Services (Shanghai, China). Isopropyl cyclohexane-3-ene carboxylate (CHCI) and methyl cyclohex-3-ene-1-carboxylate (CHCM) were purchased from Haohong Scientific Co. Ltd (Shanghai, China). XAD-22 resin was purchased from ROHM (USA). D101, D201, AB-8 and D301 resins were purchased from Anhui Sanxing (China). DEAE resin was purchased from Ruidahenghui (China). DAG standard was purchased from Yuanye Biotechnology (China). All other chemicals were analytical reagents.

All chemicals and reagents, such as zirconium
oxychloride octahydrate (ZrOCl₂·8H₂O, ≥99.0%,
Aladdin), 1,3,5-benzenetricarboxylic acid (H₃BTC, ≥96.0%,
Macklin), N,N-dimethylformamide (DMF, ≥99.5%,
Macklin), formic acid (CH₂O₂, ≥98.0%,
Macklin), poly(vinylidene fluoride) (PVDF, Macklin), poly(vinylpyrrolidone)
(PVP, Aladdin), imidazole (C₃H₄N₂, ≥99.0%, Macklin), titanium tetrachloride (TiCl₄, ≥99.0%, Macklin), 4-nitrophenol (C₆H₅NO₃, ≥99.5%, Macklin), dimethyl chlorophosphate
((CH₃O)₂P(O)Cl, 96.0%, Aladdin), triethylamine
(C₆H₁₅N, ≥99.0%, Aladdin), tetrahydrofuran
(C₄H₈O, ≥99.9%, Aladdin), ethyl acetate
(C₄H₈O₂, ≥99.5%, Aladdin), n-hexane (C₆H₁₄, ≥98.0%, Aladdin),
acetone (C₃H₆O, ≥99.8%, Merckmillipore),
magnesium sulfate anhydrous (MgSO₄, ≥99.9%, Macklin),
chloroform-d (CDCl₃, 99.8%, LaboTecc),
methyl sulfoxide-d₆ (DMSO-d₆, 99.9%, Macklin) and sulfuric acid-d₂ solution (D₂SO₄, 99.5%, Aladdin)
were obtained from available commercial sources and used without further
purification. DMNP was synthesized by following the reported procedure,^{38 (link)} and the details were described in the Supporting Information.