Primary antibodies used in this study can be found in Appendix Table S3 . Secondary antibodies used for wide‐field microscopy were purchased from Thermo‐Fisher Scientific and included: goat anti‐rabbit, anti‐mouse or anti‐guinea pig conjugated to Alexa Fluor 350, 488, 594 or 647 (1:500 dilution); donkey anti‐rabbit, anti‐mouse or anti‐goat conjugated to Alexa 488, 555 and 594 (1:500 final dilution). For STED microscopy, anti‐rabbit Atto594 (1:100 dilution) and anti‐mouse Star635p (1:100 dilution) were purchased from Sigma and Abberior, respectively. Secondary antibodies for immunoblotting were horseradish peroxidase conjugated goat anti‐rabbit or anti‐mouse antibodies (1:1,000 dilution; purchased from Jackson ImmunoResearch).
Anti mouse star635p
Manufactured by Merck Group
Anti‐mouse Star635p is a fluorescent dye that can be used to label antibodies or other proteins for detection in immunofluorescence applications. It has a bright emission spectrum and is compatible with standard red fluorescence detection systems.
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2 protocols using anti mouse star635p
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Antibodies Used in Microscopy and Immunoblotting
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Antibody Labeling and Detection
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Primary antibodies used in this study can be found in Table S4. Secondary antibodies used for wide-field microscopy were purchased from Thermo-Fisher Scientific and included: goat anti-rabbit, anti-mouse or anti-guinea pig conjugated to Alexa Fluor 350, 488, 594 or 647 (1:500 dilution); donkey anti-rabbit, anti-mouse or anti-goat conjugated to Alexa 488, 555 and 594 (1:500 final dilution). For STED microscopy, anti-rabbit Atto594 (1:100 dilution) and anti-mouse Star635p (1:100 dilution) were purchased from Sigma and Abberior, respectively. Secondary antibodies for immunoblotting were horseradish peroxidase conjugated goat anti-rabbit or anti-mouse antibodies (1:1000 dilution) (purchased from Jackson ImmunoResearch).
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