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2 protocols using trisodium citrate dihydrate

1

Characterization of Trace Metals in Edible Salts

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Silver nitrate was purchased from Jingming Chemical, Taiwan. Sodium hydroxide, citric acid monohydrate, trisodium citrate dihydrate, and magnesium sulfate anhydrous were purchased from SHOWA, Japan. Sodium borohydride, copper (II) nitrite trihydrate, and sodium nitrite were purchased from ACROS, USA. Glycerol and iron (III) nitrate nonahydrate were purchased from Alfa Aesar, USA. Hg (II) nitrate hydrate and nickel (II) nitrate hexahydrate were purchased from VETEC, USA. Lead (II) nitrate was purchased from J.T. Baker, USA. Nitric acid and hydrochloric acid were purchased from AENCORE, Australia. The mercury standard solution (1000-μg/mL Mercury AA Standard Solution) was purchased from AccuStandard, New Haven, CT, USA.
Edible salts such as Unification of Life sun-dried sea salt (origin: Spain), Taiyen Biotech iodized high-quality salt (origin: Taiwan), and Marsel natural fine sea salt (origin: Belgium) were purchased from Carrefour, Taiwan. Taiyen Biotech healthy iodized sea salt (origin: Taiwan), Unification of Life sun-dried iodized sea salt (origin: Melbourne, Australia), and Natural Salt New Zealand sun-dried natural sea salt (origin: Marlborough, New Zealand) were purchased from PX mart, Taiwan. Kirkland Signature pure sea salt (origin: Australia) was purchased from Costco Wholesale Corporation, Taiwan.
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2

Oxidative Stress Measurement Protocol

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All regents were analytical purity and used without further purification. Potassium hexacyanoferrate(III) (K3[Fe(CN)6], 99%) was bought from Riedel-de-Haën. Trisodium citrate dihydrate (C6H5Na3O7‧2H2O, 99%) was obtained from SHOWA. Hydrochloric acid (HCl, 36%) was bought from BASF. Manganese(II) acetate (C4H6MnO4, 98%), and tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride complex (Ru(ddp)3Cl2, C72H48Cl2N6Ru) were obtained from Alfa Aesar. Ethanol (C2H5OH, 99.9%) was purchased from J. T. Baker. Aminophenyl fluorescein solution (APF, C26H17NO5, 98%) was acquired from Life Technologies. Cobalt(II) acetate (C4H6CoO4, 99%), Nickel(II) acetate tetrahydrate (C4H6NiO4‧4H2O, 98%), Polyvinylpyrrolidone (PVP, (C6H9NO)n, M.W. = 55,000), hydrogen peroxide solution (H2O2, 30%), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, C44H84NO8P, 99%), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE, C41H78NO8P, 99%), potassium iodide (KI, 99.5%), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, C18H16BrN5S, 97.5%) were bought from Sigma-Aldrich. Water was obtained by using a Millipore direct-Q deionized water system throughout all studies.
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