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Anti por

Manufactured by Abcam

Anti-Por is a laboratory reagent used to detect the presence of proteins. It serves as a tool for protein identification and quantification in various biological samples. The product's core function is to facilitate the visualization and analysis of proteins in research and diagnostic applications.

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2 protocols using anti por

1

Immunofluorescence Staining of Cryopreserved Tissue

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Example 8

10 μm sections from cryopreserved tissue blocks were fixed with 3% PFA for 15 minutes and incubated overnight at 4° C. with the following primary antibodies: anti-Por (Abcam, cat #ab13513) diluted 1:500, anti-human Nuclei (EMD Millipore, cat #MAB 1281) diluted 1:250 in PBS containing 0.2% TritonX-100 and 0.5% BSA. Secondary antibodies (1:1,000 Alexa-fluor conjugated, Molecular Probes) were incubated for 60 min at room temperature in the same buffer. Sections were mounted with Vectashield plus DAPI (Vector Labs).

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2

Cryosectioning and Immunostaining of Tissue

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Ten-micrometer sections from cryopreserved tissue blocks were fixed with 3% PFA for 15 min and incubated overnight at 4 °C with the following primary antibodies: anti-Por (Abcam, cat# ab13513) diluted 1:500, anti-human Nuclei (EMD Millipore, cat# MAB1281) diluted 1:250 in PBS containing 0.2% Triton X-100, and 0.5% BSA. Secondary antibodies (1:1,000 Alexa-fluor conjugated, Molecular Probes) were incubated for 60 min at room temperature in the same buffer. Sections were mounted with Vectashield plus DAPI (Vector Labs).
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