Example 8
10 μm sections from cryopreserved tissue blocks were fixed with 3% PFA for 15 minutes and incubated overnight at 4° C. with the following primary antibodies: anti-Por (Abcam, cat #ab13513) diluted 1:500, anti-human Nuclei (EMD Millipore, cat #MAB 1281) diluted 1:250 in PBS containing 0.2% TritonX-100 and 0.5% BSA. Secondary antibodies (1:1,000 Alexa-fluor conjugated, Molecular Probes) were incubated for 60 min at room temperature in the same buffer. Sections were mounted with Vectashield plus DAPI (Vector Labs).