Cells were cultivated fixed with BD cytofix/cytoperm fixation/permeabilization kit (cat. no. 554715 BD Biosciences; Franklin Lakes, NJ, USA) at room temperature for 20 min and then stained with fluorescent 488-phalloidin (green) for actin using the manufacturer’s recommendations (Molecular Probes/Invitrogen Life Technologies). Fluorescence images were collected on Zeiss LSM-700 confocal microscope. For the analysis of the cellular internalization of exosomes, Zeiss acquisition parameters, including exposure, focus, illumination, and Z stack projection, were controlled by Zen 2012 Imaging Software for Acquisition and Analysis.
Zen 2012 imaging software for acquisition and analysis
Manufactured by Zeiss
Zen 2012 Imaging Software is a comprehensive software package designed for image acquisition and analysis. It provides a robust and user-friendly interface for controlling Zeiss microscopes and capturing high-quality images. The software offers a range of tools and features for image processing, analysis, and visualization, enabling researchers to efficiently manage and extract valuable information from their microscopy data.
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Lab products found in correlation
2 protocols using zen 2012 imaging software for acquisition and analysis
1
Actin Cytoskeleton Visualization
2
Actin Cytoskeleton Visualization and Exosome Internalization
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Cells were cultivated fixed with BD cytofix/cytoperm™ fixation/permeabilization Kit (Cat. No. 554715 BD bioscience, Franklin Lakes, NJ, USA) at room temperature for 20 min and then stained with fluorescent 488‐Phalloidin (green) for actin using the manufacturer's recommendations (Molecular Probes/Invitrogen Life Technologies). Fluorescence images were collected on Zeiss LSM‐700 confocal microscope. For the analysis of the cellular internalization of exosomes Zeiss acquisition parameters, including exposure, focus, illumination, and Z stack projection, were controlled by Zen 2012 Imaging Software for Acquisition and Analysis.
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