Mir 195 5p inhibitor

Human melanoma A375 cells, M14 cells and HEK 293T cells were all purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and cultured in DMEM (Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin (Thermo Fisher Scientific), at 37°C in a 5% CO₂ incubator. MiR-497-5p mimics, miR- 497-5p inhibitor, miR-195-5p mimics, miR-195-5p inhibitor, miR-455-3p mimics, miR-455-3p inhibitor, mimics negative control (mimics NC) and inhibitor negative control (inhibitor NC) were synthesized and purchased from GenePharma Co, Ltd (Shanghai, China). The sequences are listed in Table S3. Human melanoma A375 cells with the concentration of 2×10⁵ cells per well were seeded in 6-well plates and cultured for 24 h. Then miRNAs mimics/inhibitors/NC (50 nM) were transfected into the A375 cells using Lipofectamine 2000 (Thermo Fisher Scientific) in serum-free medium according to the manufacturer’s instructions.