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Flow set beads

Manufactured by Beckman Coulter
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Flow set beads are a type of laboratory equipment used in flow cytometry applications. They are designed to calibrate and validate the performance of flow cytometers, which are instruments used to analyze and sort cells or particles in a fluid suspension. The flow set beads are typically composed of uniform, fluorescently-labeled particles that are used to ensure the accuracy and precision of flow cytometry measurements.

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Lab products found in correlation

Versacomp antibody capture beads, by Beckman Coulter (1 mentions) Flow check pro beads, by Beckman Coulter (1 mentions) Fc500 cytometer, by Beckman Coulter (1 mentions) Cxp software, by Beckman Coulter (1 mentions)

Spelling variants of this product

Flow-Set Pro Beads (5 citations) Flow‐Set (2 citations) Flow-Set Fluorospheres beads (2 citations)

2 protocols using flow set beads

1

Flow Cytometry Instrument Maintenance

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Daily cleaning of the flow cell fluidics line was performed using filtered Isoflo and Clenz® (Beckman Coulter, USA). For daily monitoring of instrument performance, flow set beads (Beckman Coulter, USA) and flow check pro beads (Beckman Coulter, USA) were used and plotted on a levy Jennings plot. Antibody titrations were performed to determine the optimal antibody concentrations and colour compensation was performed using the versa comp antibody capture beads (Beckman Coulter, USA).
Davison G.M., Nkambule B.B., Mkandla Z., Hon G.M., Kengne A.P., Erasmus R.T, & Matsha T.E. (2017). Platelet, monocyte and neutrophil activation and glucose tolerance in South African Mixed Ancestry individuals. Scientific Reports, 7, 40329.
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2

Lymphocyte Subset Analysis in Cancer Radiotherapy

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Blood was collected two times before and 4 weeks after the initiation of CRT. Lymphocyte subpopulations (CD3+ T lymphocyte, CD3+/CD4+ helper T lymphocytes, CD3+/CD8+ cytotoxic T lymphocyte, CD3-/CD19+ B lymphocytes, and CD3-CD56+ natural killer cell [NK cell]) counts (cells/μL) and CD4+/CD8+ ratio were analyzed by multicolor flow cytometry. Acquired cell were analyzed by flow cytometry using a FC-500 cytometer (Beckman Coulter Inc., Brea, CA, USA). Monoclonal antibodies were used for cell surface staining. Instrument setting parameters such as threshold were set with machine software (CXP Software; Beckman Coulter Inc.) in conjunction with calibration beads (Flow-Set beads, CYTO-COMP kit, and CYTO-TROL Control Cells). Data were analyzed with CXP analysis software.
Heo J., Oh Y.T., Noh O.K., Chun M., Park J.E, & Cho S.R. (2016). Nodal tumor response according to the count of peripheral blood lymphocyte subpopulations during preoperative chemoradiotherapy in locally advanced rectal cancer. Radiation Oncology Journal, 34(4), 305-312.
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