Pex 3 mettl3 expression plasmid

Manufactured by GenePharma

Sourced in China

The PEX-3-METTL3 expression plasmid is a laboratory tool designed for the expression of the METTL3 gene. METTL3 is an enzyme involved in the post-transcriptional modification of RNA. The plasmid contains the necessary genetic elements to enable the expression of the METTL3 protein in various cell lines or experimental systems.

Automatically generated - may contain errors

Lab products found in correlation

Si ythdf1, by GenePharma (2 mentions) Lentivirus for, by GenePharma (2 mentions) Simettl3 1, by GenePharma (2 mentions) Simettl3 2, by GenePharma (2 mentions) Pcdna3.1 slc7a11 expression plasmid, by Genomed (2 mentions) Sh mettl3, by GenePharma (2 mentions)

2 protocols using pex 3 mettl3 expression plasmid

Targeted Knockdown and Overexpression of METTL3

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The siRNAs for METTL3 and YTHDF1, and lentivirus for METTL3 knockdown were synthesized by GenePharma (Shanghai, China). The sequences were as follows: siMETTL3#1 (sense: 5′-GCUACCUGGACGUCAGUAUTT-3′, antisense: 5′-AUACUGACGUCCAGGUAGCTT-3′); siMETTL3#2 (sense: 5′-GGUUGGUGUCAAAGGAAAUTT-3′, antisense: 5′-AUUUCCUUUGACACCAACCTT-3′); siYTHDF1 (sense: 5′-GGAGAAUAACGACAACAAATT-3′, antisense: 5′-UUUGUUGUCGUUAUUCUCCTT-3′); and shMETTL3 (5′-GCAAGAATTCTGTGACTATGG-3′).
The pEX-3-METTL3 expression plasmid was synthesized by GenePharma (Shanghai, China). The pcDNA3.1-SLC7A11 expression plasmid was synthesized by Genomeditech (Shanghai, China).

Xu Y., Lv D., Yan C., Su H., Zhang X., Shi Y, & Ying K. (2022). METTL3 promotes lung adenocarcinoma tumor growth and inhibits ferroptosis by stabilizing SLC7A11 m6A modification. Cancer Cell International, 22, 11.

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METTL3 and YTHDF1 RNA Silencing Constructs

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The siRNAs for METTL3 and YTHDF1, and lentivirus for METTL3 knockdown were synthesized by GenePharma (Shanghai, China). The sequences were as follows:
siMETTL3#1 (sense: 5′-GCUACCUGGACGUCAGUAUTT-3′, antisense: 5′-AUACUGACGUCCAGGUAGCTT-3′); siMETTL3#2 (sense: 5′-GGUUGGUGUCAAAGGAAAUTT-3′, antisense: 5′-AUUUCCUUUGACACCAACCTT-3′); siYTHDF1 (sense: 5′-GGAGAAUAACGACAACAAATT-3′, antisense: 5′-UUUGUUGUCGUUAUUCUCCTT-3′); and shMETTL3 (5'-GCAAGAATTCTGTGACTATGG-3').
The pEX-3-METTL3 expression plasmid was synthesized by GenePharma (Shanghai, China). The pcDNA3.1-SLC7A11 expression plasmid was synthesized by Genomeditech (Shanghai, China).

Xu Y., Lv D., Yan C., Su H., Zhang X., Shi Y., & Ying K. (2021). METTL3 Promotes Lung Adenocarcinoma Tumorigenesis and Inhibits Ferroptosis by Stabilizing SLC7A11 m6A Modification.

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