The extraction of tissue protein in ipsilateral hemispheres tissues was performed following the instructions of the reagents kit and using RIPA buffer. The concentrations of protein were detected by the BCA Protein Assay Kit (Sigma-Aldrich). After divorced by the SDS-PAGE, the protein samples were transferred to a PVDF filter membrane. Following being blocked with 5% nonfat milk, primary antibodies (anti-phosphorylated nNOS at Ser1214, Sigma-Aldrich; anti-3-nitrotyrosine, Abcam; anti-matrix metalloproteinase-9 (MMP-9), Abcam; ZO-1, Invitrogen, USA; NLRP3, Abcam; IL-1β, Abcam) were incubated respectively and followed by incubation with species-specific secondary antibodies (goat anti-mouse IgG 1:2000, Santa Cruz Biotechnology; goat anti-rabbit IgG 1:2000, Santa Cruz Biotechnology). Densitometry analysis was performed with Image J software after normalization to GAPDH.
Anti matrix metalloproteinase 9 mmp 9
Manufactured by Abcam
Sourced in United States
Anti-matrix metalloproteinase-9 (MMP-9) is a laboratory product that can be used to detect and measure the presence of MMP-9 in various samples. MMP-9 is an enzyme involved in the breakdown of extracellular matrix proteins and plays a role in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Merck Group (1 mentions)
Goat anti rabbit igg, by Santa Cruz Biotechnology (1 mentions)
Goat anti mouse igg, by Santa Cruz Biotechnology (1 mentions)
Anti 3 nitrotyrosine, by Abcam (1 mentions)
Il 1β, by Abcam (1 mentions)
Nlrp3, by Abcam (1 mentions)
Ripa buffer, by Thermo Fisher Scientific (1 mentions)
Anti collagen 1, by Abcam (1 mentions)
Anti collagen 3, by Affinity Biosciences (1 mentions)
2 protocols using anti matrix metalloproteinase 9 mmp 9
1
Protein Extraction and Western Blot Analysis
2
Protein Expression Analysis by Western Blot
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Proteins were extracted by RIPA buffer (Invitrogen) and separated by 10% SDS-PAGE gels, transferred onto the Polyvinylidene Fluoride (PVDF) membrane, and probed with the appropriate primary antibodies specific for anti-cytokeratin 10 (CK10; Affinity), anti-cytokeratin 14 (CK14; Affinity),anti-Matrix metalloproteinase-9 (MMP9; Abcam), anti-Collagen-I(Abcam), anti-Collagen-III(Affinity) at 4°C overnight, and goat anti-mouse secondary antibody at 37°C for 50 min. The primary antibodies included Fluorescence scanning imaging. ImageJ software was used to analyze and process the strips.
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