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Apc ef780 conjugated anti human cd31

Manufactured by Thermo Fisher Scientific
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The APC-eF780 conjugated anti-human CD31 is a flow cytometry reagent that binds to the CD31 antigen expressed on the surface of human cells. It can be used to identify and enumerate cells expressing CD31, which is commonly associated with endothelial cells.

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Lab products found in correlation

Pe conjugated anti human cd144, by BioLegend (2 mentions) Percp ef710 conjugated anti human cd43, by Thermo Fisher Scientific (2 mentions) Hoechst 33258, by Thermo Fisher Scientific (2 mentions) Stemspan, by STEMCELL (2 mentions) Flt3l, by STEMCELL (1 mentions) Methocult sf h4636, by STEMCELL (1 mentions) Flt3l, by Thermo Fisher Scientific (1 mentions) Il 11, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

APC-conjugated anti-CD31 APC-conjugated CD31 Anti-CD31 APC Anti-human CD31 CD31-APC EF780 Anti-CD31

2 protocols using apc ef780 conjugated anti human cd31

1

Endothelial Progenitor Cell Isolation

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After 6 days of culture, EBs were collected, disaggregated, and stained with APC-eF780 conjugated anti-human CD31 (Thermo Fisher Scientific), PE-conjugated anti-human CD144 (BioLegend), PerCP-eF710-conjugated anti-human CD43 (Thermo Fisher Scientific) and Hoechst 33258 (Thermo Fisher Scientific). Live CD31+CD144+CD43- cells were sorted and replated for in StemSpan (STEMCELL Technologies) supplemented with 5ng/ml VEGF, 5ng bFGF, 25ng/ml IGF1, 25ng/ml IGF2, 50ng/ml SCF, 50ng/ml TPO, 5ng/ml IL-11, 20ng/ml Flt3-L (all cytokines from PeproTech). Colony forming units (CFU) were performed using MethoCult™ SF H4636 (Stem Cell Technologies).
Menegatti S., Potts B., Paredes R., Garcia-Alegria E., Baker S.M, & Kouskoff V. (2023). CD82 expression marks the endothelium to hematopoietic transition at the onset of blood specification in human. iScience, 26(9), 107583.
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2

Endothelial Progenitor Cell Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols
After 6 days of culture, EBs were collected, disaggregated and stained with APC-eF780 conjugated anti-human CD31 (Thermo Fisher Scientific), PE-conjugated anti-human CD144 (BioLegend), PerCP-eF710-conjugated anti-human CD43 (Thermo Fisher Scientific) and Hoechst 33258 (Thermo Fisher Scientific). Live CD31+CD144+CD43− cells were sorted and replated on gelatin-coated dishes in StemSpan (STEMCELL Technologies) supplemented with 5 ng/ml VEGF, 5ng bFGF, 25 ng/ml IGF1, 25 ng/ml IGF2, 50 ng/ml SCF, 50 ng/ml TPO, 5 ng/ml IL-11, 20 ng/ml Flt3-L (all cytokines from PeproTech). Additional materials and methods are available in Supplemental Text.
Menegatti S., Potts B., Garcia-Alegria E., Paredes R., Lie-A-Ling M., Lacaud G, & Kouskoff V. (2021). The RUNX1b Isoform Defines Hemogenic Competency in Developing Human Endothelial Cells. Frontiers in Cell and Developmental Biology, 9, 812639.
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