Cell light edu apollo in vitro imaging kit

Glioma cells were planted in 96-well plates at the density of 2500 cells per well for 24 hours. Cell viability rate was determined using the Cell Counting Kit-8 (Dojindo, Japan). Optical density value was measured at 450 nm. Each group set five replicated wells and CCK-8 assay was performed in three independently repeated experiments. EDU immunocytochemistry staining was performed by Cell-Light™ EdU Apollo In Vitro Imaging Kit (Ribobio, Guangzhou, China)

Cell proliferation was determined using a Cell Counting Kit-8 (CCK-8) assay kit (Dojindo, Japan) and a cell-light 5-ethynyl-2′-deoxyuridine (EdU) Apollo Imaging Kit (Ribobio, Guangzhou, China). For the CCK-8 assay, U87 and U251 cells were seeded into 96-well plates for 0, 24, 48, and 72 h at a density of 3000 cells per well. Then, 10 μL CCK-8 solution was added to each well and incubated with the cells for 2 h. Absorbance was detected at 450 nm using a microplate reader (Bio-Rad, Hercules, CA, USA). EdU immunocytochemistry staining was performed by using a Cell-Light™ EdU Apollo In Vitro Imaging Kit (Ribobio, Guangzhou, China) at 24 h after the cell was plated into 96-well plates. The EdU-positive cells were visualized under a fluorescence microscope (Olympus, Tokyo, Japan).

Cell Counting Kit-8 (CCK8, Dojindo, Kumamoto, Japan), colony formation, and EdU assays were used to analyze cell growth capacity, as described in our previous study [14 (link)]. The EdU assay was performed with the Cell-Light EdU Apollo in vitro Imaging Kit (Ribobio, Guangzhou, China) according to the manufacturer’s instructions.