We induced apoptosis by treating the cells with camptothecin (6 μM, Sigma-Aldrich) for 4 hrs. Apoptotic cells were measured using propidium iodide (PI) and an Annexin V-FITC binding assay kit II (BD PharMingen, San Diego, CA, USA), according to the manufacturer’s protocol. The apoptotic cells were analysed using a FACScan (Becton Dickinson).
Annexin 5 fitc binding assay kit 2
Manufactured by BD
Sourced in United States
The Annexin V-FITC binding assay kit II is a laboratory tool used to detect and quantify apoptotic cells. It utilizes the binding properties of Annexin V, a calcium-dependent phospholipid-binding protein, which has a high affinity for phosphatidylserine (PS) residues that are exposed on the surface of apoptotic cells. The kit includes Annexin V conjugated with the fluorescent dye FITC, which allows for the visualization and analysis of apoptotic cells using flow cytometry or fluorescence microscopy.
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Lab products found in correlation
2 protocols using annexin 5 fitc binding assay kit 2
1
Apoptosis Induction and Quantification
2
Quantifying Apoptosis by Flow Cytometry
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We induced apoptosis by treating the cells with camptothecin (6 μM, Sigma-Aldrich, St-louis, MO, USA for 4 h. Apoptotic cells were measured using propidium iodide (PI) and an Annexin V-FITC binding assay kit II (BD PharMingen, San Diego, CA, USA), according to the manufacturer’s protocol. The apoptotic cells were analyzed using a FACScan (Becton Dickinson).
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