Released N-glycans were desalted using a Superdex™ Peptide™ 3.2/30 size exclusion column on a Beckman™ Gold chromatograph. Desalted N-glycans were analyzed in triplicate using negative mode hydrophilic interaction liquid chromatography (HILIC) -MS with a constant acetonitrile post-column make-up-flow on an Agilent™ 6520 LC-MS system with chip-cube™ nanoESI source, as described previously (32 (link)).
6520 lc ms system
Manufactured by Agilent Technologies
The 6520 LC-MS system is a high-performance liquid chromatography-mass spectrometry (LC-MS) instrument designed for analytical applications. It provides accurate mass measurement and high-resolution separation capabilities. The system is capable of performing qualitative and quantitative analysis of a wide range of compounds.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 6520 lc ms system
1
N-Glycan Desalting and HILIC-MS Analysis
2
N-Glycan Purification and Analysis
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