Immunostains were performed on all cases and controls using steam antigen retrieval and standard techniques. Immunostains were performed for Keratin 7 (1:100; Clone OV-TL 12/30; Dako; CA), CD68 (1: 1500; Clone KP1; Dako, CA), Arginase (predilute; Clone SP156; Cell Marque, CA) and PRKAR1A (1:2000; Clone OTi6C7; Origene, MD).
Clone oti6c7
Manufactured by OriGene
The Clone OTi6C7 is a laboratory equipment product that functions as a molecular biology tool. It is designed for use in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Clone ov tl 12 30, by Agilent Technologies (2 mentions)
Clone kp1, by Agilent Technologies (1 mentions)
Hep par 1, by Roche (1 mentions)
Cd68 clone kp1, by Agilent Technologies (1 mentions)
L fabp, by Abcam (1 mentions)
C reactive protein (crp), by Abcam (1 mentions)
Prkar1a, by OriGene (1 mentions)
2 protocols using clone oti6c7
1
Immunohistochemical Markers for Characterization
2
Immunohistochemistry and FISH Analysis of Liver Tumors
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Five micron-thick sections were cut from the tissue block and immunohistochemistry performed using antibodies to Arginase-1(predilute, clone SP156; Cell Marque), HepPar1 (predilute, clone OCH1E5; Ventana), cytokeratin 7 (1:100, Clone OV-TL 12/30; Dako), CD68 (clone KP1, 1:50-1:100; Dako), PRKAR1A (1:2000, clone OTI6C7; Origene Technologies), LFABP (1:50, rabbit polyclonal; Abcam), CRP (1:2000, clone Y284; Abcam), SAA (1:2000, clone mc1; Dako), Glutamine synthetase (1:2000, clone GS-6; Millipore), and beta catenin (predilute, clone 14; Ventana). Steam antigen retrieval and standard clinical methods were used for each antibody.
Fluorescence in situ Hybridization (FISH) FISH for PRKACA rearrangement was performed on 5 µ-thick formal-fixed sections. PRKACA FISH was performed on the hepatic adenomas and the paired fibrolamellar carcinomas. In addition, we examined the untested fibrolamellar carcinomas in the followup cohort (n = 8). PRKACA FISH used the validated probe and methodology as described previously. 1 FISH slides were visualized and scored by two experienced technologists who each independently evaluated 50 tumor nuclei.
Fluorescence in situ Hybridization (FISH) FISH for PRKACA rearrangement was performed on 5 µ-thick formal-fixed sections. PRKACA FISH was performed on the hepatic adenomas and the paired fibrolamellar carcinomas. In addition, we examined the untested fibrolamellar carcinomas in the followup cohort (n = 8). PRKACA FISH used the validated probe and methodology as described previously. 1 FISH slides were visualized and scored by two experienced technologists who each independently evaluated 50 tumor nuclei.
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