Ripa lysis buffer r0010

Glycyrrhizic acid (DG0006) was obtained from Chengdu DeSiTe Biological Technology Co., Ltd (China). Zinc sulfate (A602906) and galactose (A600215) were purchased from Sangon Biotech (China). PEI (E8420), Hemotoxylin and Eosin staining kit (G1120) and RIPA lysis buffer (R0010) were purchased from Solarbio Life Science (China). DSPE-PEG 2000 was produced by Xi'an Ruixi Biological Technology Co., Ltd. (China). Penicillin–streptomycin, fetal bovine serum (FBS) and Dulbecco's modified Eagle’s medium (DMEM) were produced by Gibco (USA). Free fatty acids (1170460001), palmitic acid (P0500) and Oil Red O (O0625) were obtained from Sigma (USA). p-IKKβ (Abcam, ab59195, 1:1000), IKKβ (Abcam, ab124957, 1:1000), p-p65 (ab86299), p65 (ab16502), TNF-α (ab183218), IL-6 (ab259341), ABCA1 (ab66217), CPT1 (ab234111) primary antibodies and horseradish peroxidase (HRP)-labeled secondary antibodies were obtained from Abcam (USA).

Recombinant human Trop2 protein (10428-H08H) was purchased from Sino Biological (Guangzhou, China). DAPI (ZLI-9557) was obtained from ZSGB-Bio (Beijing, China). TMB substrate (PA107) was obtained from Tian Gen (Beijing, China). A CCK-8 assay kit (CK04) was purchased from Dojindo (Tokyo, Japan). StemXVivo Tumor Sphere Media (CCM012) was obtained from R&D Systems (Minnesota, USA). The BCA protein assay kit (PC0020) and RIPA lysis buffer (R0010) were obtained from Solarbio (Beijing, China). Cell cycle kits (CCS012) and Annexin V-FITC/PI apoptosis kits (AP101) were purchased from Multi Sciences (Zhejiang, China). Verapamil (S4202) was obtained from Selleck.cn. Vybrant^TM DyeCycle^TM Violet Ready Flow^TM (DCV, R37172) were purchased from Thermo Fisher Scientific (Waltham, USA). Cell culture medium was obtained from Basal Media (Shanghai, China), and Fetal Bovine Serum (FBS) was purchased from VivaCell Biosciences (Shanghai, China).