Quantikine high sensitivity elisa kit hs600b

At age 38, plasma IL-6 (pg/mL) was measured on a Molecular Devices (Sunnyvale, CA) SpectraMax plus 384 plate reader using R&D Systems (Minneapolis, MN) Quantikine High-sensitivity ELISA kit HS600B according to the manufacturer’s instructions. At age 45, serum IL-6 (pg/mL) was measured on a Cobas e 602 analyzer (Roche Diagnostics GmbH), using an electrochemiluminescence immunoassay. The lower detection limit of the assay was 1.5 pg/mL. The intraassay and interassay CVs reported by the manufacturer were 2.5–6.0% and 2.9–8.5%, respectively. IL-6 increased on average 0.6 pg/mL from age 38 to 45, with changes ranging from −17.3 to 27.6 pg/mL. IL-6 values were log-transformed to account for positive skew. Transformed IL-6 levels were correlated at age 38 and 45, r = .45, p < .001.

Systemic inflammation markers included CRP, fibrinogen, E-selectin, and IL-6. Their concentrations were determined using fasting blood samples drawn on the second day of the clinical visit, before breakfast, at approximately 7 am. The collection and processing of the samples followed standardized procedures to ensure consistency. Fibrinogen concentrations (mg/dL) in citrated plasma were measured using immunoturbidometric assay at the University of Wisconsin, Coe Lab. CRP concentrations (ug/mL) in citrated plasma were measured using immunoturbidometric assay at the University of Vermont, Tracy Lab. BNII nephelometer from Dade Behring utilizing a particle enhanced immunonepholometric assay was used. Serum levels of soluble E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, were measured in ng/mL at the University of Vermont, Tracy Lab. High sensitivity ELISA assay (Parameter Human sESelectin Immunoassay; R&D Systems, Minneapolis, MN) was utilized. IL-6 concentrations (pg/mL) in serum were determined using the Quantikine^® High-sensitivity ELISA kit #HS600B (R&D Systems, Minneapolis, MN) at the University of Vermont, Tracy Lab.