MODE-K cells (Mus musculus, small IECs, kindly provided by D. Kaiserlian) were cultured in high-glucose DMEM (Lonza, BE12-604F), 10% FCS (Biochrome, S0115), HEPES (10 mM, Biochrome, L1613), non-essential amino acids (1 mM, Gibco, 11140-035), 100U/ml penicillin and 100 µg/ml streptomycin (1% Biochrome, 0257 F). HEK293T cells (Homo sapiens, female, embryonic kidney cells, ATCC, CRL-1573) were cultured in high-glucose DMEM (Lonza, BE12-604F), 10% FCS (Biochrome, S0115), HEPES (10 mM, Biochrome, L1613), non-essential amino acids (1 mM, Gibco, 11140-035), 100U/ml penicillin and 100 µg/ml streptomycin (1% Biochrome, 0257 F) and 2 mM sodium pyruvate (1% Biochrome, L 0473). Cells were cultured at 37 °C in 5% CO2.
Mayr L., Grabherr F., Schwärzler J., Reitmeier I., Sommer F., Gehmacher T., Niederreiter L., He G.W., Ruder B., Kunz K.T., Tymoszuk P., Hilbe R., Haschka D., Feistritzer C., Gerner R.R., Enrich B., Przysiecki N., Seifert M., Keller M.A., Oberhuber G., Sprung S., Ran Q., Koch R., Effenberger M., Tancevski I., Zoller H., Moschen A.R., Weiss G., Becker C., Rosenstiel P., Kaser A., Tilg H, & Adolph T.E. (2020). Dietary lipids fuel GPX4-restricted enteritis resembling Crohn’s disease. Nature Communications, 11, 1775.
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