Anti keratin 5

For histology, tissues were placed in zinc formalin for 18–24 hours and then formalin was replaced with 70% ethanol (Sigma). Formalin-fixed tissues were embedded in paraffin and sectioned into 5^m sections, deparaffinized, and rehydrated, followed by Hematoxylin & Eosin staining (H&E) and immunofluorescence staining. Immunofluorescence was performed via heat- mediated antigen retrieval with a Tris-EDTA buffer (10mM Tris, 1mM EDTA, pH 9.0) in a Retriever 2100 pressure cooker (Electron Microscopy Sciences). Slides were incubated overnight at 4°C with the following primary antibodies: anti-vimentin (abcam, ab24525, 1:100 dilution), anti-CD45 (Cell Signaling, 13917, 1:100), anti-CD31 (Cell Signaling, 3528, 1:250), and anti-keratin 5 (LSBio, LS-C22715, 1:500). Alexa Fluor-conjugated secondary antibodies were used to detect the primary antibodies (ThermoFisher, Alexa 488, 546, 594, & 647, all secondary antibodies at 1:300 dilution), and nuclei stained with DAPI (ThermoFisher, 1μg/mL) prior to covering with ProLong Gold Antifade Mountant (ThermoFisher). Slides were imaged on the Leica TCS SP8 X (Leica) located in the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) Light Imaging Core and processed on LAS X software (Leica).