Fresh leaves of 4-week-old MicroTom that were grown in half-strength Murashige and Skoog basal medium with sucrose and Phytagel were collected, and the genomic DNA was sequenced on the Nanopore platform using a MinION R9 flow cell and the Illumina NovaSeq6000 platform with an insert size of 450 bp at Benagen (Wuhan, China).
Fresh tissues of roots, stems, leaves, flower, immature green fruits, and mature red fruits of plants grown in nutrient-rich soil were subjected to total RNA extraction using TRIzol (Invitrogen, Carlsbad, USA) according to the manufacturer’s protocol, and were used for subsequent RNA sequencing using different strategies. Samples from each tissue were subjected to construct a ribo-minus RNA library and small RNA library for RNA sequencing, whereas a mixture of the six samples was used to construction a PacBio SMRT library and a circular RNA library. The ribo-minus RNA libraries, small RNA libraries, and circular RNA library were sequenced using the Illumina HiSeq platform at Novogene Co., Ltd (Tianjin, China), and the PacBio SMRT library was sequenced using the PacBio Sequel System at Novogene Co., Ltd (Tianjin, China).
Fresh tissues of roots, stems, leaves, flower, immature green fruits, and mature red fruits of plants grown in nutrient-rich soil were subjected to total RNA extraction using TRIzol (Invitrogen, Carlsbad, USA) according to the manufacturer’s protocol, and were used for subsequent RNA sequencing using different strategies. Samples from each tissue were subjected to construct a ribo-minus RNA library and small RNA library for RNA sequencing, whereas a mixture of the six samples was used to construction a PacBio SMRT library and a circular RNA library. The ribo-minus RNA libraries, small RNA libraries, and circular RNA library were sequenced using the Illumina HiSeq platform at Novogene Co., Ltd (Tianjin, China), and the PacBio SMRT library was sequenced using the PacBio Sequel System at Novogene Co., Ltd (Tianjin, China).