Micromass q tof ultima global gaa076 lc mass spectrometer

Specific rotations were obtained on a JASCO P-1020 digital polarimeter. UV spectra were recorded on a HITACHI 5430. IR spectra were measured on a Bruker Tensor-27 spectrophotometer in KBr discs. NMR spectra were recorded on a JEOLJN M-ECP 600 spectrometer (JEOL, Tokyo, Japan) or an Agilent 500 MHz DD2 spectrometer using tetramethylsilane as an internal standard. HRMS were obtained on a Thermo Scientific LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) or Micromass Q-TOF ULTIMA GLOBAL GAA076 LC mass spectrometer (Waters Corporation, Milford, MA, USA). Semipreparative HPLC was performed on an ODS column (YMC-Pack ODS-A, 10 × 250 mm, 5 μm, 3 mL/min).

NMR spectra were recorded on a JEOLJN M-ECP 600 spectrometer (JEOL, Tokyo, Japan) or Bruker Avance Neo 400 MHz (Bruker, Beijing, China) using tetramethylsilane as an internal standard. Specific rotations were obtained on a JASCO P-1020 digital polarimeter. UV spectra were recorded on HITACHI 5430. IR spectra were measured on a Bruker Tensor-27 spectrophotometer in KBr discs. HRESIMS were obtained on a Thermo Scientific LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) or Micromass Q-TOF ULTIMA GLOBAL GAA076 LC mass spectrometer (Waters Corporation, Shanghai, China). A JASCO J-715 spectropolarimeter (JASCO, Tokyo, Japan) was used to obtain ECD spectra. Semipreparative HPLC was performed on an ODS column (YMC-Pack ODS-A, 10 × 250 mm, 5 μm, 3 mL/min).

Optical rotations were measured with a JASCO P-1020 digital polarimeter (JASCO Corporation, Tokyo, Japan). UV spectra were recorded on a Beckman DU 640 spectrophotometer (Beckman Coulter, Inc., Miami, USA). IR spectra were recorded on a Nicolet Nexus 470 spectrophotometer (Thermo Fisher Scientific Inc., Massachusetts, USA) in KBr discs. HR-ESI-MS spectra were measured on a Micromass Q-TOF Ultima Global GAA076 LC mass spectrometer (waters corporation, Milford, USA). NMR spectra were recorded on Bruker 400 MHz spectrometers (Bruker Daltonics Inc., Karlsruhe, Germany) using TMS (Tetramethyl silane) as an internal standard δ. X-ray diffraction data were collected on an Agilent Technologies Gemini A Ultra system (Agilent Technologies Inc., Palo Alto, USA). Semipreparative HPLC was performed on an Agilent 1260 LC series with a DAD detector using an Agilent Eclipse XDB-C₁₈ column (250 × 10.0mm, 5 µm). Silica gel (300-400 mesh, Qingdao Marine Chemical Inc., China), Silica gel (200-300) mesh (Qingdao Marine Chemical Inc., China), Silica gel H (10-40 μm, Qingdao Marine Chemical Inc., China), Lichroprep RP-18 gel (40-63 μm, Merck, Darmstadt, Germany), and Sephadex LH-20 (40-70 μm, Amersham Biosciences, Sweden) were used for column chromatography (CC).