Murine Th1 and Th17 frequency were quantified in mouse splenocytes cultured in 0.25µg/ml of CD3 Ab and CD28 Ab (Biolegend) and cells were untreated (PBS) or treated with IL-12 (10ng/ml; Th1+Ctl, Biolegend), TGFβ+IL-6 (1+20ng/ml respectively; Th17+Ctl, Biolegend) or LPS (100ng/ml) in presence or absence of IACS (100nM) for 4 days and supernatants were measured for cytokine secretion by ELISA. Thereafter, following APC-conjugated CD4 Ab (eBioscience) staining, cells were fixed and permeabilized and splenocytes were stained with FITC-conjugated IFNγ or PE-labeled IL‐17 Ab (eBioscience). RA monocytes-differentiated into MΦs for 3 days were treated with PBS or LPS/IFNγ (100ng/ml each) for 24h before staining with FITC-labeled CD14 Ab (Biolegend), APC-conjugated CD86 (Biolegend) or PC7-labeled CD206 (Biolegend). Cells were gated based on the unstained and the gating strategy is provided in Supplementary F and G .
Th1 ctl
Manufactured by BioLegend
Th1+Ctl is a laboratory product offered by BioLegend. It serves as a positive control for the detection and quantification of Th1 cells. The product consists of a population of cells that express markers characteristic of the Th1 cell subset.
Automatically generated - may contain errors
Lab products found in correlation
Cd3 ab and cd28 ab, by BioLegend (2 mentions)
Th17 ctl, by BioLegend (2 mentions)
Apc conjugated cd4 ab, by Thermo Fisher Scientific (2 mentions)
Fitc conjugated ifnγ, by Thermo Fisher Scientific (2 mentions)
Pe labeled il 17 ab, by Thermo Fisher Scientific (2 mentions)
Fitc labeled cd14 ab, by BioLegend (2 mentions)
Pc7 labeled cd206, by BioLegend (2 mentions)
Apc conjugated cd86, by BioLegend (2 mentions)
2 protocols using th1 ctl
1
Th1/Th17 Differentiation and Macrophage Polarization
2
Th1/Th17 Differentiation and Macrophage Polarization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Murine Th1 and Th17 frequency were quantified in mouse splenocytes cultured in 0.25µg/ml of CD3 Ab and CD28 Ab (Biolegend) and cells were untreated (PBS) or treated with IL-12 (10ng/ml; Th1+Ctl, Biolegend), TGFβ+IL-6 (1+20ng/ml respectively; Th17+Ctl, Biolegend) or LPS (100ng/ml) in presence or absence of IACS (100nM) for 4 days and supernatants were measured for cytokine secretion by ELISA. Thereafter, following APC-conjugated CD4 Ab (eBioscience) staining, cells were fixed and permeabilized and splenocytes were stained with FITC-conjugated IFNγ or PE-labeled IL‐17 Ab (eBioscience). RA monocytes-differentiated into MΦs for 3 days were treated with PBS or LPS/IFNγ (100ng/ml each) for 24h before staining with FITC-labeled CD14 Ab (Biolegend), APC-conjugated CD86 (Biolegend) or PC7-labeled CD206 (Biolegend). Cells were gated based on the unstained and the gating strategy is provided in Supplementary F and G .
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