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Anti pd 1 pe ebioj105

Manufactured by Thermo Fisher Scientific
Sourced in United States

Anti-PD-1-PE (eBioJ105) is a fluorescently labeled antibody that binds to the programmed cell death-1 (PD-1) receptor. It is a flow cytometry reagent used for the detection and analysis of PD-1 expression on cells.

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2 protocols using anti pd 1 pe ebioj105

1

Rhesus Macaque Immune Cell Profiling

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The following monoclonal antibodies (mAbs) that cross-reacted with rhesus macaque were obtained from BD Pharmingen (BD Biosciences, CA, USA): anti-CD3-PE/-APC-Cy7 (clone SP34-2), anti-CD4-FITC/-PerCP-Cy5.5 (clone L200), anti-CD8α-PE-Cy7 (clone RPA-T8), anti-CD20-PerCP-Cy5.5 (clone 2H7), anti-CD14-APC (clone M5E2), anti-HLA-DR-APC (clone L243), anti-CD28-APC (clone CD28.2), anti-CD95-FITC (clone DX2), anti-Ki67-PE (clone B56), and anti-IL-4-PerCP-Cy5.5 (clone 8D4-8). Anti-CD38-FITC (clone AT-1) mAb was obtained from STEMCELL. Anti-PD-1-PE (eBioJ105) was obtained from eBioscience (CA, USA). Anti-IL-2-FITC (clone MQ1-17H12), anti-IFN-γ-PE (clone 4S.B3), and anti-TNF-α-APC (clone Mab11) mAbs were all obtained from BioLegend (CA, USA).
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2

Multiparametric Flow Cytometry Profiling

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Human mAbs that cross-react with rhesus macaques were used at their optimal concentrations in this study. Anti-CD38-FITC (clone AT-1) was obtained from StemCell Technologies. Anti-PD-1-PE (eBioJ105) and anti-CD27-PE-Cy5 (O323) were obtained from eBioscience. Anti-CCR5-PE (3A9), anti-CD14-APC (M5E2), anti-CD28-APC (CD28.2), anti-CD20-APC-H7/FITC (2H7), anti-CD3-APC-Cy7 (SP34-2), anti-CD4-PerCP-Cy5.5 (L200), anti-CD8-PE-Cy7 (RPA-T8), anti-CD80-FITC (L307.4), anti-CD86-PE (FUN-1), anti-CD95-FITC/PE-Cy7 (DX2), anti-CXCR4-PE-Cy7 (12G5), anti-HLA-DR-APC (G46-6) and anti-Ki67-PE (B56) were all obtained from BD Biosciences. Polyclonal anti-IgD-biotin was obtained from Southern Biotech, and streptavidin-PE-Cy7 was obtained from BioLegend.
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