Goat antimouse trop2

3-month-old and 24-month-old mouse prostate tissue was embedded in paraffin and sectioned at UCLA’s Translational Pathology Core Laboratory. Sections were incubated at 60°C in a vacuum oven for 45-60 min. Slides were transferred into xylene (Fisher) 3 times, 100% alcohol (Decon Labs) 2 times, 95% ethanol 1 time and 70% ethanol 1 time, each for 3 min. Slides were transferred into PBS (GIBCO) for 5 min prior to epitope unmasking using a heat antigen retrieval step. Staining of sections was performed using the manufacturer’s protocol for the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (R & D Systems) with primary antibody goat antimouse Trop2 (R & D Systems) at a 10 μg/ml concentration. Hematoxylin and eosin staining was performed by UCLA’s Translational Pathology Core Laboratories. Frozen sections were fixed with 4% paraformaldehyde in PBS for 5 min at room temperature, washed with PBS, and stained with primary antibodies and stained with the following secondary antibodies: goat anti-mouse IgG-Alexa Fluor 488 and goat anti-rabbit IgG-Alexa Fluor 594.