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Histopaque 1119 1083 1077 gradient

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Histopaque 1119/1083/1077 gradient is a density gradient medium used for the isolation of mononuclear cells from whole blood. It consists of a polysucrose and sodium diatrizoate solution with a specific density that allows for the separation of blood components during centrifugation.

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Lab products found in correlation

Ultra sensitive insulin homogeneous time resolved fluorescence assay kits, by PerkinElmer (2 mentions) Accutase, by Merck Group (2 mentions) Facsaria 3 flow cytometer, by BD (2 mentions) Liberase, by Roche (2 mentions)

Spelling variants of this product

Histopaque-1077 (1979 citations) Histopaque (690 citations) Histopaque-1083 (278 citations) Histopaque 1119 (267 citations) Histopaque gradient (60 citations) Histopaque-1077 gradient (33 citations) Histopaque 1077/1119 (14 citations) Histopaque-1083 gradient (7 citations)

2 protocols using histopaque 1119 1083 1077 gradient

1

Islet Isolation and Purification

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To isolate primary islets, the pancreas was inflated with Liberase TM (Roche, UK), digested at 37°C and purified using a Histopaque 1119/1083/1077 gradient (Sigma-Aldrich, UK), and islets were hand-picked. For FACS-based experiments, cells from purified islets were isolated using Accutase (Sigma-Aldrich) and sorted using a FACSAria III flow cytometer (BD Biosciences, UK), as detailed in the ESM Methods. Total insulin content was assessed using ultra-sensitive insulin homogeneous time-resolved fluorescence (HTRF) assay kits (Cisbio, France).
Yu V., Yong F., Marta A., Khadayate S., Osakwe A., Bhattacharya S., Varghese S.S., Chabosseau P., Tabibi S.M., Chen K., Georgiadou E., Parveen N., Suleiman M., Stamoulis Z., Marselli L., De Luca C., Tesi M., Ostinelli G., Delgadillo-Silva L., Wu X., Hatanaka Y., Montoya A., Elliott J., Patel B., Demchenko N., Whilding C., Hajkova P., Shliaha P., Kramer H., Ali Y., Marchetti P., Sladek R., Dhawan S., Withers D.J., Rutter G.A, & Millership S.J. (2024). Differential CpG methylation at Nnat in the early establishment of beta cell heterogeneity. Diabetologia, 67(6), 1079-1094.
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2

Isolation and Purification of Primary Islets

Check if the same lab product or an alternative is used in the 5 most similar protocols
To isolate primary islets, the pancreas was inflated with Liberase TM (Roche), digested at 37°C, purified using a Histopaque 1119/1083/1077 gradient (Sigma-Aldrich) and hand-picked. For FACS-based experiments, cells from purified islets were isolated using Accutase (Sigma-Aldrich) sorted using an FACSAria III flow cytometer (BD, UK). Total insulin content was assessed using ultra-sensitive insulin homogeneous time-resolved fluorescence (HTRF) assay kits (Cisbio).
Yu V., Yong F., Marta A., Khadayate S., Osakwe A., Bhattacharya S., Varghese S.S., Chabosseau P., Tabibi S.M., Chen K., Georgiadou E., Parveen N., Suleiman M., Stamoulis Z., Marselli L., De Luca C., Tesi M., Ostinelli G., Delgadillo-Silva L., Wu X., Hatanaka Y., Montoya A., Elliott J., Patel B., Demchenko N., Whilding C., Hajkova P., Shliaha P., Kramer H., Ali Y., Marchetti P., Sladek R., Dhawan S., Withers D.J., Rutter G.A, & Millership S.J. (2023). Differential CpG methylation at Nnat in the early establishment of beta cell heterogeneity. bioRxiv.
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