After BMSC transplantation, the rats were examined via MRI (Siemens Verio 3.0T MRI system, Erlangen, Bavaria Land, Germany) at 1-, 2-, 3- and 4-week post-transplantation. The scanning sequence included T1-weight images (T1WI), T2-weighted images (T2WI), T2-fluid-attenuated inversion recovery (FLAIR), and multiple B-value diffusion-weighted imaging (Wang et al., 2011). After the original IVIM image was transformed with the ImageJ software (National Institutes of Health, Bethesda, MD, USA), the brain region of interest was identified in the T1WI and applied to all other image types. Information from each region of interest was manually plotted and measured; each region of interest was measured three times using four individual elements. The animals were re-examined one week later, and the average values of the repeated measurements were evaluated using the F value (perfusion fraction) and D value (pure diffusion coefficient). The D value represents the diffusion movement of pure water (slow diffusion movement), which excludes the influence of microcirculation perfusion associated with the capillary network on diffusion in the tissue structure. More restricted diffusion of water molecules corresponded with a lower D value (Federau et al., 2014).
Verio 3.0t mri system
Manufactured by Siemens
Sourced in Germany
The Verio 3.0T MRI system is a magnetic resonance imaging (MRI) equipment manufactured by Siemens. It operates at a field strength of 3.0 Tesla, which allows for high-quality image acquisition. The core function of the Verio 3.0T MRI system is to generate detailed images of the body's internal structures and organs using strong magnetic fields and radio waves.
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Lab products found in correlation
3 protocols using verio 3.0t mri system
1
Tracking BMSC Transplantation via MRI
2
MRI Assessment of Liver and Cardiac T2*
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(1) Detection of liver and cardiac T2* by MRI: using verio 3.0T MRI system of Siemens company in Germany, the image acquisition of liver and cardiac was completed by skilled operators. Routine abdominal scan included fat suppression T2* WI in coronal and transverse axial positions, dual echo T1* WI in the same/ reverse phase and diffusion-weighted imaging sequence. After routine scanning, the multi echo gradient echo sequence was used to collect liver axial multi echo T2* WI, and patients were asked to hold their breath at the end of breath during scanning.
Imaging parameters: echo time(TE)(2.0-11.8)ms, echo interval 0.6 ms, total 16 echoes, repetition time (TR)200 ms, layer thickness 10 mm, scanning time 17 s. Then, myocardial T2* imaging was performed, and two chamber and four chamber localization images were collected in turn. The short axis position of the heart was selected at the place with the largest transverse diameter of the interventricular septum at the four chamber level, and multi echo gradient echo sequence was used. During the scanning process, the patients were asked to hold their breath at the end of exhalation.
Imaging parameters: echo time(TE)(2.0-11.8)ms, echo interval 0.6 ms, total 16 echoes, repetition time (TR)200 ms, layer thickness 10 mm, scanning time 17 s. Then, myocardial T2* imaging was performed, and two chamber and four chamber localization images were collected in turn. The short axis position of the heart was selected at the place with the largest transverse diameter of the interventricular septum at the four chamber level, and multi echo gradient echo sequence was used. During the scanning process, the patients were asked to hold their breath at the end of exhalation.
3
Comprehensive MRI Imaging Protocols
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The Siemens Verio 3.0-T MRI system used in this study (Siemens; Munich, Germany). Post-MRI processing and image evaluation were carried outin a syngo R MR WorkPlace (Siemens) workstation. A LF Optistar TM LE double-syringe power injector (Mallinckrodt Pharmaceuticals; St Louis, MO) was used. The MRI scanning sequence included conventional T 1 WI, T 2 WI, fluid-attenuated inversion recovery (FLAIR), DWI, SWI, and PWI, with a test time of about 12 minutes. Scanning parameters for T 1 WI, T 2 WI, T 1 -FLAIR, DWI, and SWI included TR = 1500 ms, TE = 30 ms, FA = 90 • , slice thickness = 4.0 mm, slice gap = 5.2 mm, FOV = 230 × 100 mm, and slice number = 950 (single selection). A total of 900 consecutive images were selected from 60 groups. Magnevist (Gd-DTPA; Bayer Schering, Leverkusen, Germany) was used as the MRI contrast agent at a dosage of 0.1 mmol/kg. A bolus injection was administered using the power injector at a rate of 4 ml/s.
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