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Cytopeia influx

Manufactured by BD
Sourced in Australia

The Cytopeia Influx is a flow cytometry instrument designed for cell analysis and sorting. It is capable of detecting and measuring multiple parameters of individual cells within a heterogeneous population.

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2 protocols using cytopeia influx

1

Flow Cytometric Analysis and Cell Sorting

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Flow cytometric analysis was done using a LSRII and cell sorting used a Cytopeia Influx (BD Biosciences, Sydney, Australia) equipped as previously described [32 (link)]. For generic analysis, cells were analysed at 10–20,000 cells per second except for cell cycle analysis which were analysed at 2000 cells per second. Cell sorting was performed at ~20,000 cells per second and re-analysed to confirm purity (>95%).
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2

Flow Cytometric Analysis of R-BC154

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Flow cytometric analysis was performed using an LSR II (BD Biosciences)16 (link). R-BC154 was detected at either 582 or 610 nm and excited with the yellow-green laser (561 nm). For BM and PB analysis cells were analysed at 10–20,000 cells per s. Cell sorting was performed on a Cytopeia Influx (BD)16 (link). All sorts were re-analysed with a purity of >98%.
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