Mnova 9

Manufactured by Mestrelab Research

MNova 9.0 is a software application developed by Mestrelab Research for the analysis and processing of nuclear magnetic resonance (NMR) data. The software provides tools for handling and visualizing NMR spectra, as well as performing common data processing tasks such as baseline correction, peak integration, and multiplet analysis.

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Lab products found in correlation

Topspin 3, by Bruker (2 mentions) Amx 400, by Bruker (1 mentions)

Close spelling variants of this product

Mnova software 9.0 MNova

2 protocols using mnova 9

NMR Characterization of Compounds

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NMR spectra were recorded in CD 3 OD at 400 MHz for 1 H and 100 MHz for 13 C using a Bruker Avance III HD 400 instrument equipped with a 5 mm BBFO probe (MPICE), Bruker Avance II 400 instrument equipped with a 5 mm BBFO probe (NPAC, UniNE), or at 700 MHz for 1 H and 175 MHz for 13 C using a Bruker Avance III HD 700 instrument equipped with a 1.7 mm TCI microcryoprobe (MPICE). Residual solvent signals with 1 H at 3.31 ppm and 13 C at 49.05 ppm were used as internal standard. Two-dimensional homonuclear double quantum filtered (dqf )-COSY spectra were recorded using phase cycling for coherence selection. For the isolated compounds a total of 32 scans were acquired using a time domain of 8k in F2 (acquisition time of 1.2 s) and 512 increments in F1. Spectra were zero-filled prior to Fourier transformation, phased, and baseline corrected using the Topspin 3.2 (Bruker) and MNova 9.0 (Mestrelab Research) software.

Dong C., Dolke F., Bandi S., Paetz C, & von Reuß S.H. (2020). Dimerization of conserved ascaroside building blocks generates species-specific male attractants in Caenorhabditis nematodes. Organic & biomolecular chemistry, 18(27).

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NMR Spectroscopy Analysis Protocol

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NMR spectra were recorded in CD₃OD or CDCl₃ at 400 MHz for ¹H and 100 MHz for ¹³C using a Bruker AMX400 instrument. Residual solvent signals were used as internal standard with ¹H at 3.31 ppm and ¹³C at 49.05 ppm for CD₃OD or ¹H at 7.26 ppm and ¹³C at 77.16 ppm for CDCl₃. Two-dimensional homonuclear double quantum filtered (dqf)-COSY spectra were recorded using phase cycling for coherence selection. For the isolated compounds a total of 32 scans were acquired using a time domain of 8k in F2 (acquisition time of 1.2 s) and 512 increments in F1. For two-dimensional heteronuclear HSQC spectra 96 scans were acquired using a time domain of 1k in F2 and 256 increments in F1. Spectra were zero-filled to 8k × 4k (COSY) or 4k × 2k (HSQC) prior to Fourier transformation, phased manually, and baseline corrected using the Topspin 3.2 (Bruker) and MNova 9.0 (Mestrelab Research) software.

Dong C., Reilly D.K., Bergame C., Dolke F., Srinivasan J, & von Reuss S.H. (2018). Comparative Ascaroside Profiling of Caenorhabditis Exometabolomes Reveals Species-Specific (ω) and (ω − 2)-Hydroxylation Downstream of Peroxisomal β-Oxidation. The Journal of organic chemistry, 83(13), 7109-7120.

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