All cell lines were maintained at 37 °C under humidified conditions and 5% CO2. The N9 cells were maintained in RPMI (Corning, Corning, NY, USA) with 10% calf serum (Atlanta Biologicals, Flowery Branch, GA, USA). The HMC3 cells were maintained in DMEM medium (Corning, Corning, NY, USA) supplemented with 10% calf serum. CHO cells were maintained in DMEM:F-12 medium (Corning, Corning, NY, USA) 1:1 with 10% calf serum. SHSY5Y cells were maintained in DMEM:F12 (Corning, Corning, NY, USA) 1:1 with 10% calf serum and non essential amino acids (Thermo Fisher Scientific, Waltham, MA, USA).
Calf serum
Manufactured by Corning
Sourced in United States
Calf serum is a cell culture media supplement derived from the blood of calves. It provides a complex mixture of proteins, growth factors, and other nutrients essential for the growth and maintenance of various cell types in vitro. The core function of calf serum is to support the proliferation and viability of cultured cells.
Automatically generated - may contain errors
Lab products found in correlation
Dmem f12 medium, by Corning (1 mentions)
Calf serum, by Atlanta Biologicals (1 mentions)
Dmem f12, by Corning (1 mentions)
Roswell park memorial institute (rpmi), by Corning (1 mentions)
Dmem medium, by Corning (1 mentions)
Non essential amino acids (neaa), by Corning (1 mentions)
Mts kit, by Promega (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
2 protocols using calf serum
1
Cell Culture Maintenance Protocols
2
Immunological Effects of PAMK and Cyclophosphamide in Geese
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PAMK (purity 70%) was purchased from Tianyuan (Xi'an, China), diluted with water and sprayed on the feed according to the concentration of 400 mg kg−1. Cyclophosphamide (Jiangsu Hengrui Pharmaceutical, China), was dissolved in physiological saline and intraperitoneally injected in the geese at a concentration of 40 mg kg−1 BW. Lymphocyte separation fluid was purchased from Tianjin Haoyang Biological Manufacturers. Phytoagglutinin (PHA; Sigma, USA), as the T cell mitogen, was dissolved in 15 μg mL−1 with RPMI-1640. Calf serum (Corning, USA) was used to culture lymphocytes and an MTS Kit (Promega, USA) was used to detect the T cell proliferation rate. The enzyme-linked immunosorbent assay (ELISA) kit was purchased from Nanjing Jiancheng Bioengineering Institute. TRIzol reagent (Invitrogen, China) and a first-strand complementary (cDNA) kit (Invitrogen, China) were used for quantitative real-time PCR analysis.
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