Elizabethkingia miricola peptide n glycosidase f

Manufactured by Promega

Elizabethkingia miricola peptide-N-glycosidase F is an enzyme that cleaves the bond between an asparagine residue and the first N-acetylglucosamine of N-linked glycoproteins.

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Lab products found in correlation

Ltq velos pro ion trap mass spectrometer, by Thermo Fisher Scientific (1 mentions)

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Peptide-N-glycosidase F (7 citations)

2 protocols using elizabethkingia miricola peptide n glycosidase f

Comprehensive N-glycan Profiling by LC-MS/MS

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N-glycans were released from nMPO using Elizabethkingia miricola peptide-N-glycosidase F (Promega) (70 (link)). Reduced N-glycans were profiled in technical triplicates using porous graphitised carbon (PGC) liquid chromatography–tandem mass spectrometry (LC-MS/MS) in negative ion polarity on an LTQ Velos Pro ion trap mass spectrometer (Thermo Scientific) (71 (link)). Glycan fine structures were manually elucidated (72 (link)). RawMeat v2.1 (Vast Scientific) and GlycoMod (Expasy) aided the process. N-glycans were quantified from area-under-the-curve (AUC) measurements of extracted ion chromatograms (EICs) using Skyline v20.1.0.76 (72 (link)), see Extended experimental procedures for details.

Tjondro H.C., Ugonotti J., Kawahara R., Chatterjee S., Loke I., Chen S., Soltermann F., Hinneburg H., Parker B.L., Venkatakrishnan V., Dieckmann R., Grant O.C., Bylund J., Rodger A., Woods R.J., Karlsson-Bengtsson A., Struwe W.B, & Thaysen-Andersen M. (2020). Hyper-truncated Asn355- and Asn391-glycans modulate the activity of neutrophil granule myeloperoxidase. The Journal of Biological Chemistry, 296, 100144.

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N-Glycan Profiling by PGC LC-MS/MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

N-glycans were released from nMPO using Elizabethkingia miricola peptide-N-glycosidase F (Promega) (Jensen et al., 2012) . Reduced N-glycans were profiled in technical triplicates using porous graphitised carbon liquid chromatography-tandem mass spectrometry (LC-MS/MS) in negative ion polarity on an LTQ Velos Pro ion trap mass spectrometer (Thermo Scientific) (Hinneburg et al., 2019) . Glycan fine structures were manually elucidated (Ashwood et al., 2018) . RawMeat v2.1 (Vast Scientific) and GlycoMod (Expasy) aided the process. N-glycans were quantified from area-under-the-curve (AUC) measurements of extracted ion chromatograms (EICs) using Skyline v20.1.0.76 (Ashwood et al., 2018) .

Tjondro H.C., Ugonotti J., Kawahara R., Chatterjee S., Loke I., Chen S., Soltermann F., Hinneburg H., Parker B.L., Venkatakrishnan V., Dieckmann R., Grant O.C., Bylund J., Rodger A., Woods R.J., Karlsson A., Struwe W.B., & Thaysen‐Andersen M. (2020). Hyper-Truncated Glycans Augment the Activity of Neutrophil Granule Myeloperoxidase.

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