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Anti mmp 3

Manufactured by Bioworld Technology
Sourced in United States

Anti-MMP-3 is a laboratory reagent used for the detection and quantification of MMP-3 (matrix metalloproteinase-3), an enzyme involved in various biological processes. This product provides a specific and sensitive way to measure MMP-3 levels in samples.

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3 protocols using anti mmp 3

1

Protein Expression Analysis Protocol

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Fetal bovine serum (FBS) was purchased from Millipore (Billerica, MA, USA). High-glucose DMEM medium was purchased from Hyclone (Logan, Utah, USA). Rabbit anti-C-Myc, rabbit anti-Cyclin D1 rabbit anti-β-catenin and APC monoclonal antibodies were purchased from Cell Signaling (Danvers, MA, USA), Rabbit anti-MMP-9 monoclonal antibody was purchased from Millipore (Billerica, MA, USA), Rabbit anti-ZEB1 monoclonal antibody was purchased from Abcam (Eugene, USA), and anti-MMP-3 and anti-β1-actin monoclonal antibodies were purchased from Bioworld (Shanghai, China).
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2

Xenograft Model of Gastric Cancer

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Fetal bovine serum (FBS): Millipore (Billerica, MA, USA); High-glucose DMEM medium: Hyclone (Logan, Utah, USA). Rabbit anti-C-Myc, anti-Cyclin D1, anti-β-catenin and APC monoclonal antibodies: Cell Signaling (Danvers, MA, USA); Rabbit anti-MMP-9 antibody: Millipore (Billerica, MA, USA); Rabbit anti-ZEB1 antibody: Abcam (Eugene, USA); anti-MMP-3 and anti-β-actin monoclonal antibodies: Bioworld (Shanghai, China).
Animal experiments 20g nude mice were subcutaneously injected with 5×10 6 SGC-7901/DDP cells with miR-200b mimics or NC lentivirus. After 2 weeks, the tumor size wad observed and measured. At the 3rd week, the mice were sacri ced by neck dragging. Tumors were photographed and weighed. All experimental protocols used on the animals were approved by the institutions' subcommittees on animal care of Anhui Medical University.
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3

Xenograft Model of Gastric Cancer

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Fetal bovine serum (FBS): Millipore (Billerica, MA, USA); High-glucose DMEM medium: Hyclone (Logan, Utah, USA). Rabbit anti-C-Myc, anti-Cyclin D1, anti-β-catenin and APC monoclonal antibodies: Cell Signaling (Danvers, MA, USA); Rabbit anti-MMP-9 antibody: Millipore (Billerica, MA, USA); Rabbit anti-ZEB1 antibody: Abcam (Eugene, USA); anti-MMP-3 and anti-β-actin monoclonal antibodies: Bioworld (Shanghai, China).
Animal experiments 20g nude mice were subcutaneously injected with 5×10 6 SGC-7901/DDP cells with miR-200b mimics or NC lentivirus. After 2 weeks, the tumor size wad observed and measured. At the 3rd week, the mice were sacri ced by neck dragging. Tumors were photographed and weighed. All experimental protocols used on the animals were approved by the institutions' subcommittees on animal care of Anhui Medical University.
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